镉胁迫对菊芋生理抗逆性及根际土壤酶活性的影响  

Stress Resistance and Rhizosphere Soil Enzyme Activity of Helianthus tuberosus under Cadmium Stress

作　　者：施力铭 苟秋 张航[1,2] 李然然 王丹媚 吴玉环 陈建华[1,2] 刘鹏[1,2] SHI Liming;GOU Qiu;ZHANG Hang;LI Ranran;WANG Danmei;WU Yuhuan;CHEN Jianhua;LIU Peng(Key Laboratory of Botany,Zhejiang Normal University,Jinhua,Zhejiang 321004;Key Laboratory of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province,Jinhua,Zhejiang 321004;School of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou,Zhejiang 310036,China)

机构地区：[1]浙江师范大学植物学实验室,浙江金华321004 [2]浙江省野生动物生物技术与保护利用重点实验室,浙江金华321004 [3]杭州师范大学生命与环境科学学院,浙江杭州310036

出　　处：《贵州农业科学》2018年第3期93-98,共6页Guizhou Agricultural Sciences

基　　金：国家自然科学基金项目(41571049);2016年浙江省新苗人才计划项目"镉胁迫下菊芋富集调控以及综合机理的探究"(2017R404020)

摘　　要：为菊芋综合价值的开发和环境污染的植物修复提供参考,以徐州菊芋和潍坊菊芋为材料,通过土培对其进行不同浓度的Cd2+胁迫,探究胁迫7d、14d和21d后菊芋幼苗抗氧化酶、叶绿素含量及其根际土壤酶活性的变化。结果表明:1)在胁迫初期,SOD、CAT和POD活性较对照均高,随着胁迫程度的加剧,2个菊芋品种的抗氧化酶活性总体呈先升后降趋势,但潍坊菊芋上升趋势较徐州菊芋缓慢;2)低镉浓度(T1)胁迫对菊芋叶绿素的产生有促进作用,而高镉浓度(T3)对叶绿素的积累有抑制作用,随着胁迫时间的延长,徐州菊芋的叶绿素含量呈先升后降趋势,潍坊菊芋的叶绿素含量不断降低;3)在试验初期,根际土壤的蔗糖酶、脲酶和蛋白酶活性较对照均高,其中,徐州的土壤脲酶活性最高时较对照提升40.36%,潍坊的土壤脲酶活性最高时较对照提升42.29%。随时间的推移,镉胁迫对土壤酶活性表现出低促高抑效应,菊芋可用于土壤镉污染的修复。In order to provide references for comprehensive values exploitation of Helianthus tuberosus and phytoremediation of environmental pollution,the authors used H.tuberosus in Xuzhou and H.tuberosus in Weifang as experimental materials,and put them under different Cd 2+(0 mmol/L,0.10 mmol/L,0.60 mmol/L,1.20 mmol/L)concentrations stress of soil culture to explore the changes of antioxidant enzymes,chlorophyll contents and rhizosphere soil enzyme activities of seedlings after 7,14 and 21days.Results:1)In the early stage of drought stress,SOD,CAT and POD compared with the control group showed improvement in different degree.With the increasing degree of stress,the antioxidant enzyme activity of the two kinds of H.tuberosus was generally increased first and then decreased,but the rising trend is relatively slow of H.tuberosus in Weifang;2)Low cadmium concentration had a role to the chlorophyll of H.tuberosus in promoting,and inhibited the accumulation of high concentrations of cadmium on chlorophyll.With the increase of stress time,the chlorophyll content of H.tuberosus in Xuzhou increased firstly and then decreased,the chlorophyll content of H.tuberosus in Weifang decreased continuously;3)At the beginning of the experiment,the rhizosphere soil invertase,urease and protease activity increased in varying degrees.The urease activity in Xuzhou and Weifang were enhanced by up to 40.36%and 42.29%respectively.Cadmium promoted soil enzyme activity in low concentration,while inhibited in high concentration with time.Therefore,H.tuberosus could be applied to remediation of cadmium pollution in soil.

关 键 词：菊芋 镉胁迫 抗逆性 根际土壤 酶活性 

分 类 号：S831.5[农业科学—畜牧学]