胃泌素对大鼠激素性骨坏死的治疗作用  

Therapeutic effect of gastrin on steroid-associated osteonecrosis in rats

作　　者：罗亚平 李莹莹[2] 胡纯婷 王井龙 傅国辉 陈诗慧[2] LUO Ya-ping;LI Ying-ying;HU Chun-ting;WANG Jing-long;FU Guo-hui;CHEN Shi-hui(Pathology Center,Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200080,China;Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education,Pathology Center,Shanghai Jiao Tong University College of Basic Medical Sciences,Shanghai 200025,China)

机构地区：[1]上海交通大学附属第一人民医院病理中心,上海200080 [2]上海交通大学基础医学院病理中心,细胞分化与凋亡教育部重点实验室,上海200025

出　　处：《上海交通大学学报（医学版）》2018年第3期276-280,共5页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的·探讨胃泌素对大鼠激素性骨坏死的治疗作用。方法·24只SD大鼠随机分为正常对照组(正常组)、骨坏死对照组(骨坏死组)、骨坏死治疗组(治疗组)。骨坏死组和治疗组经尾静脉注射脂多糖1次/d(600μg/kg)×2 d,同时肌内注射甲基泼尼松龙1次/d(50 mg/kg)×3 d;对照组注射等量生理盐水。甲基泼尼松龙末次注射后,治疗组腹腔注射胃泌素1次/d(800μg/kg)×14 d,骨坏死组注射等量生理盐水。治疗结束后,大鼠处死取股骨头生长板下骨小梁,通过苏木精-伊红染色法(hematoxylin-eosin staining,H-E染色法)、免疫组织化学法、荧光染色法、戈尔德染色法进行组织学分析和鉴定。结果·成功构建大鼠激素性骨坏死模型。H-E染色结果显示,治疗组较骨坏死组血栓面积、脂肪细胞数量及面积显著减少(均P<0.05);免疫组织化学试验结果显示,治疗组较骨坏死组成骨相关转录因子(Sp7)阳性细胞显著增加(P<0.01);戈尔德染色与荧光检测结果显示,治疗组较骨坏死组类骨质长度和面积明显增加(均P<0.01),骨表面骨形成率和矿化沉积速率明显升高(均P<0.01)。结论·胃泌素能够通过抑制骨髓中骨小梁内的血栓和脂肪形成、促进骨小梁的成骨来治疗大鼠激素性骨坏死。Objective·To investigate therapeutic effect of gastrin on steroid-associated osteonecrosis(SAON)in rat model.Methods·Twenty-four SD rats were randomly divided into three groups i.e.normal control group(normal group),SAON control group(SAON group)and SAON treatment group(treatment group).SAON group and treatment group were intravenously injected with lipopolysaccharide 1 time per day(600μg/kg)for 2 d and meanwhile intramuscularly injected with methylprednisolone 1 time per day(50 mg/kg)for 3 d.Normal group was injected with normal saline of the same volumns.After steroid injections,treatment group was injected with gastrin 1 time per day(800μg/kg)for 14 d,while SAON group was injected with normal saline of the same volumns.After the treatment,bone trabeculas below femoral head growth plate were dissected in the rats for bone histology.Hematoxylin-eosin(H-E)staining,immunohistochemistry,fluorescence staining and Goldner's trichrome staining were applied in this study.Results·SAON model in rats was successfully established.The result of H-E staining showed that compared with SAON group,thrombus area,number and area of fat cells in the bone marrows of treatment group obviously decreased(all P<0.05).Immunohistochemistry showed that osteogenic transcription factor(Sp7)positive cells in treatment group were more than those in SAON group(P<0.01).Compared with SAON group,osteoid length and area(Goldner′s trichrome staining),and bone formation rate and bone mineralization deposition rate(fluorescence staining)all significantly increased in treatment group(all P<0.01).Conclusion·Gastrin can effectively treat SAON in rats by suppressing thrombus and lipid formation and enhancing boneformation.

关 键 词：激素性骨坏死 胃泌素 骨组织形态计量学 大鼠 

分 类 号：R68[医药卫生—骨科学]