高糖环境对滋养层细胞腺苷单磷酸活化蛋白激酶α1亚基表达及增殖的影响  

Influence of high glucose on PRKAA1 expression and proliferation of trophoblast cells

作　　者：彭海燕 李华萍[1] PENG Hai-yan;LI Hua-ping(Department of Gynecology and Obstetrics,Shanghai Sixth People′s Hospital,Shanghai Jiao Tong University,Shanghai 200233,China)

机构地区：[1]上海交通大学附属第六人民医院妇产科,上海200233

出　　处：《上海交通大学学报（医学版）》2018年第3期299-304,共6页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市第六人民医院预研基金(LYZY-0053)~~

摘　　要：目的·研究妊娠糖尿病患者胎盘组织中的腺苷单磷酸活化蛋白激酶α1亚基(protein kinase AMP-activated catalytic subunitα1,PRKAA1)的表达水平,及体外高糖环境对滋养层细胞PRKAA1表达水平和增殖活性的影响。方法·收集妊娠糖尿病患者(19例)及同期正常妊娠孕妇(20例)的胎盘组织,分别利用实时荧光定量PCR及蛋白免疫印迹试验测定PRKAA1 mRNA及蛋白水平的表达情况。体外高糖处理滋养层细胞后,测定PRKAA1的表达;高糖培养基及PRKAA1抑制剂dorsomorphin分别作用于滋养层细胞后,利用CCK8试剂盒测定细胞增殖活性。结果·相比正常妊娠孕妇,妊娠糖尿病患者胎盘组织中PRKAA1 m RNA及蛋白表达量显著降低(均P<0.05)。体外高糖培养基处理滋养层细胞可明显降低PRKAA1的表达水平(P<0.05);高糖培养基和dorsomorphin均可抑制滋养层细胞的增殖活性(均P<0.05)。结论·妊娠糖尿病患者胎盘组织中的PRKAA1表达水平下降,体外高糖环境对滋养层细胞增殖活性的抑制作用可能是通过下调PRKAA1介导发生。Objective·To explore the expression level of protein kinase AMP-activated catalytic subunitα1(PRKAA1)in placental tissues of gestational diabetes mellitus(GDM)women,and the influence of high glucose(HG)on PRKAA1 expression and proliferation viability of trophoblast cells in vitro.Methods·The placental samples of GDM women(n=19)and normal pregnant women(n=20)of the corresponding period were collected.Real-time qPCR and Western blotting assay were used to detect the mRNA and protein levels of PRKAA1 in these biopsies,respectively.Trophoblast cells were treated by HG in vitro and then expression level of PRKAA1 was tested.CCK8 assay was used to detect proliferation viability of the cells treated by HG medium or inhibitor of PRKAA1,dorsomorphin.Results·Comparing to normal pregnant women,both mRNA and protein levels of PRKAA1 in placental tissues of GDM women significantly decreased(both P<0.05).HG treatment drastically downregulated expression of PRKAA1 in trophoblast cells in vitro(P<0.05).Both HG medium and dorsomorphin suppressed proliferation viability of trophoblast cells(both P<0.05).Conclusion·Expression level of PRKAA1 is dampened in placental tissues of GDM women.HG suppresses proliferation viability of trophoblast cells probably via downregulating PRKAA1 level in vitro.

关 键 词：妊娠糖尿病 滋养层细胞 腺苷单磷酸活化蛋白激酶α1亚基 高糖 细胞增殖 

分 类 号：R714.256[医药卫生—妇产科学]