拟南芥防御素基因PDF1.2启动子与GUS重组载体的构建与转化  

Construction and Transformation of Recombinant Plasmid of Plant Defensins Gene PDF1.2 Promoter and GUS Gene in Arabidopsis thaliana

作　　者：刘志霞 周舟[1] 蔡薇[1] 程姣[1] 任春梅[1,2] LIU Zhixia;ZHOU Zhou;CAI Wei;CHEN Jiao;REN Chunmei(College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha,Hunan 410128,China;Crop Gene Engineering Key Laboratory of Hunan Province,Changsha,Hunan 410128,China)

机构地区：[1]湖南农业大学生物科学技术学院,长沙410128 [2]作物基因工程湖南省重点实验室,长沙410128

出　　处：《作物研究》2018年第2期131-134,共4页Crop Research

基　　金：国家科技部"973"前期研究项目(2014CB160308)

摘　　要：PDF1.2基因在植物的防御系统中扮演着重要的角色,其编码产生的植物防御素参与了植物对真菌入侵、病毒感染、不良环境等逆境胁迫的防御反应。试验利用从拟南芥中通过PCR扩增和克隆的该基因启动子构建GUS报告基因的表达载体,通过根癌农杆菌浸渍转化法将表达报告基因转入拟南芥中,筛选获得了转化植株。获得以下主要结果:(1)成功克隆了拟南芥PDF1.2基因的启动子并将其与带GUS标记基因的载体进行了融合,得到了重组的融合载体;(2)成功的将带GUS标记基因的拟南芥PDF1.2基因启动子融合载体转入到拟南芥植株中并利用其自带抗性标记筛选到了抗性植株;(3)利用GUS染色技术检测了转基因植株中PDF1.2基因启动子的表达情况。PDF1.2 gene plays an important role in plant defense systems,the plant defensins encoded by PDF1.2 gene participates in plant defensive response against to adversity stress such as fungal invasion,viral infection and adverse environment conditions.The promoter of PDF1.2 gene that amplified and cloned by PCR from Arabidopsis was used to construct the expression vector of GUS reporter gene in this experiment,then the expression reporter gene was transformed into Arabidopsis through agrobacterium tumefaciens transformation method and the transformed plants was obtained by screening.The main results were showed as follows:1.The promoter of Arabidopsis PDF1.2 gene was successfully cloned and fused with the vector carrying GUS report gene to obtain a recombinant fusion vector;2.The reconstructive vector was transferred into Arabidopsis thaliana and the transgenic plants were screened successfully;3.The expression of the promoter of PDF1.2 gene in transgenic plants was detected by GUS staining.

关 键 词：载体构建 PDF1.2基因 克隆 转化 

分 类 号：Q785[生物学—分子生物学]