朱砂莲果实化学成分杀南方根结线虫活性研究  

Nematicidal Activity of Chemical Compositions from Aristolochia tuberosa Fruits against Root-knot Nematode

作　　者：卜淼淼 韩德建 董存柱 BU Miaomiao;HAN Dejian;DONG Cunzhu(Institute of Tropical Agriculture and Forestry,Hainan University,Haikou 570228,China)

机构地区：[1]海南大学热带农林学院,海口570228

出　　处：《热带亚热带植物学报》2018年第2期197-201,共5页Journal of Tropical and Subtropical Botany

基　　金：国家自然科学基金项目(31360447);海南省重点研发计划项目(ZDYF2016157)资助~~

摘　　要：为了解朱砂莲(Aristolochia tuberosa O.F.Liang et S.M.Huang)的化学成分,从其果实的甲醇提取物中分离得到4个化合物。通过波普数据分析,分别鉴定为马兜铃内酰胺W(1)、8-epidiosbulbin e acetate(2)、diosbulbin B(3)和β-sitosterol(4)。化合物1~3对南方根结线虫2龄幼虫具有不同程度的毒杀作用,尤其马兜铃内酰胺W(1)活性最好,其96 h后的LC50为119.94μg m L–1。马兜铃属植物具有开发为新型植物源杀根结线虫剂的潜在价值。In order to understand the bioactivity constituents from Aristolochia tuberosa O.F.Liang et S.M.Huang,four compounds were isolated from methanol extract of A.tuberosa fruits by using silica gel column chromatography,Sephadex LH-20 gel column chromatography,precipitation and recrystallization methods.On the basis of spectral data,their structures were identified as aristololactam W(1),8-epidiosbulbin e acetate(2),diosbulbin B(3)andβ-sitosterol(4).Furthermore,nematicidal activity of four compounds against the second stage juvenile(J2)of Meloidogyne javanica were analyzed.The compounds 1-3 exhibited obviously toxic effects on J2 of M.javanica in vitro,especially for aristololactam W with the LC50 values of 119.94μg mL–1 after 96 h.So,Aristolochia species have the potential value of developing new plant source to control root nematodes.

关 键 词：朱砂莲 果实 化学成分 南方根结线虫 杀根结线虫活性 

分 类 号：S481[农业科学—农药学]