基于超声喷泉的超声雾化结合固相萃取法提取西洋参叶中8种人参皂苷  

Extraction of Eight Ginsenosides from Leaves of Panax quinquefolium L. By Ultrasoinc Fountain-based Ultrasonic-assisted Nebulization Extraction Coupled with Solid Phase Extraction

作　　者：张培旭[1,2] 金永日 崔俐[2] 吴谦[1] 黎鹏 张寒琦 李绪文[1] ZHANG Pei-Xu;JIN Yong-Ri;CUI Li;WU Qian;LI Peng;ZHANG Han-Qi;LI Xu-Wen(College of Chemistry,Jilin University,Changchun 130021,China;Department of Neurology,First Hospital,Jilin University,Changchun,130021,China)

机构地区：[1]吉林大学化学学院,长春130021 [2]吉林大学第一医院神经内科,长春130021

出　　处：《分析化学》2018年第4期594-600,共7页Chinese Journal of Analytical Chemistry

摘　　要：实验使用超声喷泉替代气溶胶进行传质,改进了超声雾化提取-固相萃取法,设计了实验装置,并用于西洋参叶中的8种常见人参皂苷的提取。对提取剂种类、体积、pH值和盐浓度,洗脱剂种类和体积以及吸附剂质量等影响因素进行了考察与优化。在最优条件下,8种人参皂苷的回收率在96.3%~110.6%之间,RSD在2.8%~4.3%之间,本方法对8种人参皂苷均具有良好的提取率。与传统的超声雾化提取-固相萃取法相比,本方法简化了提取装置,缩短了提取时间,提高了提取效率。A new ultrasonic-assisted extraction(UANE)method coupled with solid phase extraction(SPE)using ultrasonic fountain was established for the extraction of eight common ginsenosides from leaves of Panax quinquefolium L.The extraction system has been designed and several experimental parameters,including the type and volume of extraction solvent,pH value and salt concentration of extraction solvent,type and volume of elution solvent,and amount of C 18,extraction time were examined and optimized.Under the optimal conditions,the recoveries of ginsenosides were in the range of 96.3%-110.6%,the relative standard deviations(RSDs)were in the range of 2.8%-4.3%,indicating that the method has a good performance for the extraction of these ginsenosides.Compared with traditional UANE-SPE method,the modified method simplified the extraction device,shortened the extraction time and improved the extraction efficiency.

关 键 词：超声雾化 固相萃取 超声喷泉 西洋参 人参皂苷 

分 类 号：O658.2[理学—分析化学] R284.1[理学—化学]