河床原位修复对城市河流微生物群落的影响  

Influence of riverbed in-situ remediation on microbial community in urban rivers

作　　者：李赫龙 黎双飞 李伟奇 林静 LI Helong;LI Shuangfei;LI Weiqi;LIN Jing(Shenzhen Biyuan Environmental Protection Technology Co.,Ltd.,Shenzhen 518028,China;College of Life and Ocean Sciences,Shenzhen University,Shenzhen 518060,China;Shenzhen Branch Testing Co.,Ltd.,Shenzhen 518000,China)

机构地区：[1]深圳市碧园环保技术有限公司,广东深圳518028 [2]深圳大学生命与海洋科学学院,广东深圳518060 [3]深圳市百川检测有限公司,广东深圳518000

出　　处：《环境工程技术学报》2018年第2期176-184,共9页Journal of Environmental Engineering Technology

基　　金：深圳市技术创新计划技术攻关项目(JSGG2015060117045618)

摘　　要：采用分子生物学检测方法对实施了河床原位修复的河段进行全面的微生物群落分析。结果表明:河床生态修复后微生物群落结构及多样性发生了明显的变化。从微生物多样性指数来看,河床修复段的下游大于上游;从微生物群落结构来看,上游断面中ε-变形菌纲为最主要的细菌类型,多为致病病原菌,与该采样点周边人口密集而水质未经其他处理有关。经河床原位修复后,下游断面ε-变形菌纲丰度减少,α-变形菌纲和β-变形菌纲成为最优势的类群,其他细菌比例也相对均衡,NH3-N和其他理化指标有所好转,说明微生物载体原位修复措施对城市景观河流黑臭的改善有一定的效果。An overall microbial community analysis was performed with molecular biological testing methods for the riverbed after in-situ remediation.The results showed that the microbial community structure and diversity were changed obviously after in-situ ecological remediation.In the microbial diversity index term,the lower reaches of the river after in-situ remediation were higher than the upstream reaches.In the microbial community structure term,ε-Proteobacteria were the most important bacteria in the upstream,and most of them were pathogenic bacteria,which were related to the surrounding dense population and untreated water.After in-situ remediation,ε-Proteobacteria abundance was reduced in the downstream,furthermore,α-Proteobacteria andβ-Proteobacteria became the dominant groups in the lower reaches of the river,and the proportion of other bacteria was also relatively balanced.In addition,NH 3-N and other water quality indicators were improved.The research suggested that in-situ remediation technology has obvious effect on city landscape river ecological remediation.

关 键 词：景观河流 原位修复 微生物群落 16S RDNA 

分 类 号：X522[环境科学与工程—环境工程]