一株产耐高温β-甘露聚糖酶菌株的筛选鉴定  

Screening and Identification of a Strain Highly Producing Thermostable β-Mannanase

作　　者：罗长财 缪静[2] 李国莹[1] 杜瑶[1] 余晓斌[1] LUO Zhang-Cai;MIAO Jing;LI Guo-Ying;DU Yao;YU Xiao-Bin(Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China;School of Biological Science,Ludong University,Yantai 264025,China)

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [2]鲁东大学生命科学学院,山东烟台264025

出　　处：《生物技术通讯》2018年第2期233-237,257,共6页Letters in Biotechnology

基　　金：生物技术教育部重点实验室开放基金(KLIB-KF201510);中央高校基础研究基金(111A24);111引智计划(111-2-06)

摘　　要：目的:筛选鉴定一株产耐高温β-甘露聚糖酶的天然菌株。方法:通过形态、生理生化特征及16S rDNA比对,对从水温高于68℃的热泉中分离出的一株产β-甘露聚糖酶细菌进行鉴定。结果:该菌株的最适生长温度为50℃,可在35～80℃条件下生长,确定为一种极端嗜热枯草芽胞杆菌;该菌所产的β-甘露聚糖酶可耐受90℃高温处理。结论:产耐高温β-甘露聚糖酶极端嗜热枯草芽胞杆菌的筛选鉴定,为后续重组耐高温β-甘露聚糖酶的开发奠定了基础。Objective:To screen and identify a highly thermostableβ-mannanase natural producing strain.Methods:Aβ-mannanase producing bacterium,which was isolated from a hot spring(water temperature>68℃),was identified by morphology,physiological and biochemical characteristics,and 16S rDNA analysis.Results:The bacterium could grow from 35℃to 80℃with the optimal culture temperature 50℃,and the bacterium was identified as an extreme thermophilic Bacillus subtilis.Theβ-mannanase produced by this bacterium could tolerate 90℃treatment.Conclusion:The screening and identification of the extreme thermophilic B.subtilis laid a good foundation for further development of the recombinant highly thermostableβ-mannanase

关 键 词：耐高温β-甘露聚糖酶 极端嗜热枯草芽胞杆菌 筛选 鉴定 

分 类 号：Q93-3[生物学—微生物学]