适配体在生物毒素检测中的应用进展  

Application Progress of Aptamer in Biotoxin Detection

作　　者：王耀[1,2] 李少珍[1,2] 游一 王珊[4] 李志娟 胡骁飞 王方雨[2] WANG Yao;LI Shaozhen;YOU Yi;WANG Shan;LI Zhijuan;HU Xiaofei;WANG Fangyu

机构地区：[1]河南科技大学食品与生物工程学院,食品加工与安全国家实验教学示范中心,河南洛阳471023 [2]河南省农业科学院,河南省动物免疫学重点实验室,河南郑州450002 [3]河南省农业科学院畜牧兽医研究所,河南郑州450002 [4]河南安必诺检测技术有限公司,河南郑州450001

出　　处：《河南农业科学》2018年第2期1-6,共6页Journal of Henan Agricultural Sciences

基　　金：河南省2016年财政预算项目;河南省基础与前沿项目(142300413222);河南科技大学博士科研启动基金项目(13480062);河南省高等学校重点科研项目(18B550001)

摘　　要：食物中毒是一类重要的食源性疾病,近年来由生物毒素引起的食物中毒事件频繁出现,引起了社会广泛关注,对于食品中生物毒素的快速、高效检测提出了更高要求。在众多快速检测技术中,基于适配体的检测方法是目前的研究热点之一。作为新型识别工具,适配体具有特异性强、亲和力高、制备成本低等明显优势,已被应用于食品中危害物质的识别检测。对适配体及其在各类生物毒素检测中的应用进行综述,并对该技术存在的问题及其发展方向进行探讨,以期促进基于适配体的检测方法在生物毒素快速检测中得到进一步的推广和应用。Food poisoning is an important foodborne disease.In recent years,food poisoning caused by biotoxins has been aroused the social widespread interest,thus the rapid and efficient detection of biotoxins in food has raised higher requirements.In many rapid detection techniques,the detection method based on the aptamer is one of the research focus.As a new recognition tool,aptamer has the advantages of strong specificity,high affinity and low cost of preparation.In this paper,the aptamer and its application in various biological toxin detection were reviewed,and the existing problems and development direction of the technology were discussed,so as to promote the application aptamer-based method in the rapid detection of biotoxins.

关 键 词：适配体 生物毒素 检测 

分 类 号：S859.84[农业科学—临床兽医学]