大叶蒲公英愈伤组织耐盐突变体诱导与鉴定  

Induction and Identification of Salt-tolerant Mutant of Taraxacum mongolicum Callus

作　　者：刘艳芬 王秀萍[2] 陈翠果 赵敏 梁伟玲 王婷婷[2] 董秀秀 LIU Yanfen;WANG Xiuping;CHEN Cuiguo;ZHAO Min;LIANG Weiling;WANG Tingting;DONG Xiuxiu(College of Landscape and Ecology,Hebei University of Engineering,Handan 056021,China;Key Laboratory of Plant Salt Tolerance in Tangshan City/Binhai Agricultural Research Institute,Hebei Academy of Agriculture and Forestry,Tangshan 063200,China;Agricultural and Animal Husbandry Bureau of Guangping County in Handan City,Hebei Province,Handan 057600,China)

机构地区：[1]河北工程大学园林与生态工程学院,河北邯郸056021 [2]唐山市植物耐盐研究重点实验室/河北省农林科学院滨海农业研究所,河北唐山063200 [3]河北省邯郸市广平县农牧局,河北邯郸057600

出　　处：《河南农业科学》2018年第2期102-106,共5页Journal of Henan Agricultural Sciences

基　　金：河北省科学技术与发展计划项目(16232503D-X-1);河北省科技支撑计划项目(16226304D);河北省财政专项(F15R16002)

摘　　要：为获得大叶蒲公英耐盐突变体,以其叶片为外植体,进行了愈伤组织耐盐突变体的筛选。结果表明,愈伤组织诱导和增殖的最佳培养基分别为MS+6-BA 0.5 mg/L+2,4-D 0.04 mg/L与MS+6-BA 0.4 mg/L+2,4-D 0.02 mg/L+NAA 0.2 mg/L。将继代培养5次的愈伤组织接种在含有不同质量浓度盐的诱导培养基上,经过4次耐盐胁迫,筛选出了耐盐的愈伤组织突变体。通过RAPD检测发现,在NaCl质量浓度为8、10、12 g/L的培养基上愈伤组织扩增条带与不含NaCl的培养基(对照)有差异,证明耐盐的愈伤组织中确实产生了DNA的突变。To obtain salt-tolerant mutants of Taraxacum mongolicum,using its leaves as explants the callus of salt-tolerant mutant was screened.The results indicated that the optimal medium for callus induction and proliferation was MS+6-BA 0.5 mg/L+2,4-D 0.04 mg/L and MS+6-BA 0.4 mg/L+2,4-D 0.02 mg/L+NAA 0.2 mg/L respectively.The salt-tolerant mutants were inoculated on medium with different salt concentrations,and screened out from the callus after 4 times of salt stress.Compared with the control without NaCl,there were different bands in the callus cultured on the medium with NaCl of 8,10 and 12 g/L using RAPD detection.It could be coucluded that the DNA of salt tolerant callus mutated.

关 键 词：大叶蒲公英 耐盐突变体 诱导 鉴定 

分 类 号：S647[农业科学—蔬菜学]