发酵浒苔对珍珠龙胆石斑鱼生长、非特异性免疫及消化酶活性的影响  

Effects of fermented Enteromorpha prolifera on growth performance,non-specific immunity and digestive enzyme activity of pearl gentian grouper

作　　者：徐安乐 黎中宝[1,2] 上官静波 胡晓伟 黄永春 Xu Anle;Li Zhongbao;Shangguan Jingbo;Hu Xiaowei;Huang Yongchun(Fisheries College,Jimei University,Xiamen 361021,China;Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment,Xiamen 361021,China)

机构地区：[1]集美大学水产学院,福建厦门361021 [2]福建省海洋渔业资源与生态环境重点实验室,福建厦门361021

出　　处：《海洋学报》2018年第4期96-105,共10页

基　　金：厦门市科技项目(3502Z20153009);厦门市海洋经济发展专项资金项目(14CZY045HJ19);福建省科技计划项目(2012N0018)

摘　　要：浒苔经发酵后按添加比例(0%、1%、2%、3%、4%、5%)分别加入到珍珠龙胆石斑鱼(Epinephelus lanceolatus♂×Epinephelus fuscoguttatus♀)基础饲料中配制成6种饲料,用以探讨发酵浒苔对珍珠龙胆石斑鱼生长、肠消化酶活性及血清非特异性免疫力的影响。选用初始平均体质量为(19.16±0.25)g的珍珠龙胆石斑鱼为研究材料,试验周期28d。结果表明:发酵浒苔能显著影响石斑鱼的增重率(WGR)和特定生长率(SGR)(P<0.05),且随着发酵浒苔添加水平的升高呈现先升高后降低的趋势,在2%组添加量时显著高于对照组。同时,相比于对照组,各添加组饲料系数(FCR)均有下降,在2%组时显著降低(P<0.05)。添加发酵浒苔可以显著提高血清总超氧化物歧化酶(T-SOD)、碱性磷酸酶(AKP)和酸性磷酸酶(ACP)的活性(P<0.05),但对溶菌酶(LZM)和酚氧化酶(PO)活性影响差异不显著(P>0.05)。在添加组中,淀粉酶、脂肪酶以及胰蛋白酶活性均得到了显著提高(P<0.05),添加水平在2%~3%时效果最好。综合发酵浒苔对珍珠龙胆石斑鱼的作用效果可以看出,发酵浒苔可以明显促进其生长,提高非特异性免疫力及肠道中消化酶活力,建议发酵浒苔在饲料中添加量为2%左右。To evaluate the effect of fermentation Enteromorpha prolifera on growth performance,digestive enzyme activities and serum non-specific immunity,6 diets adding 0%,1%,2%,3%,4%,5%fermentation E.prolifera were respectively fed to pearl gentian grouper(Epinephelus lanceolatus♂×Epinephelus fuscoguttatus♀)(19.16±0.25)g for 28 d.The results showed that with the increasing of fermentation E.prolifera level,the weight gain ratio(WGR)and the specific growth ratio(SGR)in each group showed a trend of first rising then falling,while the feed conversion ratio(FCR)was on the contrary.The WGR,SGR and FCR showed significantly better than the control group at the content of 2%(P<0.05).Adding fermentation E.prolifera enhanced the activities of serum total Superoxide dismutase(T-SOD),Alkaline phosphatase(AKP)and acid phosphatase(ACP)significantly(P<0.05),but no significant differences were found in the activities of Lysozyme(LZM)and Polyphenol Oxidase(PO)between trials(P>0.05).With the addition of fermentation E.prolifera,,the activity of amylase,lipase and trypsin were significantly increased(P<0.05),and the best level is 2%-3%.In conclusion,fermentation E.prolifera can obviously promote the growth performance,improved the activity of non-specific immunity enzyme in the serum and digestion enzyme in the gut,it is recommended the amount adding is about 2%.

关 键 词：发酵浒苔 珍珠龙胆石斑鱼 生长 肠道消化酶 非特异性免疫 

分 类 号：S965.334[农业科学—水产养殖]