Soybean hairy roots produced in vitro by Agrobacterium rhizogenes-mediated transformation  

作　　者：Li Chen Yupeng Cai Xiujie Liu Chen Guo Shi Sun Cunxiang Wu Bingjun Jiang Tianfu Han Wensheng Hou 

机构地区：[1]National Center for Transgenic Research in Plants, Institute of Crop Science, Chinese Academy of Agricultural Sciences [2]Ministry of Agriculture Key Laboratory of Soybean Biology (Beijing), Institute of Crop Science, Chinese Academy of Agricultural Sciences

出　　处：《The Crop Journal》2018年第2期162-171,共10页作物学报（英文版）

基　　金：supported by the Major Science and Technology Projects of China (2016ZX08010-004);the Ministry of Science and Technology of China (2016YFD0100504);the CAAS (Chinese Academy of Agriculture Sciences) Innovation Project

摘　　要：Soybean is one of the world's most important oil and protein crops. Efficient transformation is a key factor for the improvement of soybean by genetic modification. We describe an optimized protocol for the Agrobacterium rhizogenes-mediated transformation of soybean and the induction of hairy root development in vitro. Cotyledons with 0.5-cm hypocotyls were cut from 5-day-old seedlings and used as explants. After infection and co-cultivation,hairy roots were produced in induction culture medium after 10–12 days. Using this method, 90%–99% of the infected explants of five different cultivars produced hairy roots within one month. Observations using reporter constructs showed that 30%–60% of the hairy roots induced were transformed. Based on high transformation efficiency and short transformation period, this method represents an efficient and rapid platform for study of soybean gene function.Soybean is one of the world's most important oil and protein crops. Efficient transformation is a key factor for the improvement of soybean by genetic modification. We describe an optimized protocol for the Agrobacterium rhizogenes-mediated transformation of soybean and the induction of hairy root development in vitro. Cotyledons with 0.5-cm hypocotyls were cut from 5-day-old seedlings and used as explants. After infection and co-cultivation,hairy roots were produced in induction culture medium after 10–12 days. Using this method, 90%–99% of the infected explants of five different cultivars produced hairy roots within one month. Observations using reporter constructs showed that 30%–60% of the hairy roots induced were transformed. Based on high transformation efficiency and short transformation period, this method represents an efficient and rapid platform for study of soybean gene function.

关 键 词：AGROBACTERIUM RHIZOGENES HAIRY ROOT Protocol SOYBEAN Transformation 

分 类 号：S[农业科学]