阿苯达唑联合IFN-α抗小鼠细粒棘球蚴病的实验研究  

In vivo therapeutic effects of albendazole in combination with IFN-α on cystic echinococcosis in mice

作　　者：云哲琳[1] 周永升 张焱[2] 李冰[1] YUN Zhe-lin;ZHOU Yong-sheng;ZHANG Yan;LI Bing(Department of General Surgery,Baogang Hospital,Baotou 014010,China;The Sci-technology Center,Ningxia Medical University,Yinxia 750003,China)

机构地区：[1]内蒙古包钢医院普外科,包头014010 [2]宁夏医科大学科技中心,银川750003

出　　处：《中国人兽共患病学报》2018年第2期133-138,共6页Chinese Journal of Zoonoses

摘　　要：目的在BALB/c小鼠体内评价阿苯达唑(ABZ)联合干扰素(interferon,IFN)-α对于CE的治疗效果。方法Balb/c小鼠原头蚴腹腔继发感染5个月后,小鼠被随机分配到4组:ABZ组、IFN-α组、ABZ+IFN-α组和未经处理对照组。不同处理组分别给药2个月。在治疗的第0、7、14、28、36、48、60d从小鼠尾静脉采血检测血清中抗体水平变化。治疗结束后处死小鼠,检测相关指标评价治疗效果。结果与对照组(P<0.01)或ABZ组(P<0.05)相比,ABZ+IFN-α组包囊的数量、大小及重量都显著减少。对不同处理组的包囊进行透射电镜观察(TEM)发现,ABZ+IFN-α组的包囊的超微结构发生了明显的改变。ELISA实验结果表明,ABZ+IFN-α组的血清与脾细胞分泌的白细胞介素(interleukin,IL)-10显著下降(P<0.01);血清中IgE、IgG抗体及其亚型浓度与对照组相比显著降低(P<0.01)。结论本研究证实ABZ联合IFN-α可能成是一种有效的CE治疗方案。This study aimed to evaluate the in vivo efficacy of combined ABZ-interferon(IFN)-αtreatment for CE in mice.After 5 months of secondary infection with protoscoleces,mice were randomly allocated into four groups:ABZ-treated group,IFN-α-treated group,ABZ+IFN-αgroup and untreated control group.Drugs in diverse treated groups were respectively administered for 2 months,of which,sera were respectively collected in 0 d,7 d,14 d,28 d,36 d,48 d,and 60 d.Mice were then euthanized and associated indications were investigated to evaluate the therapeutic efficacy.Results showed that ABZ+IFN-αinduced a significant reduction of the number,size as well as weight of cysts,compared with that in ABZ(P<0.05)or untreated group(P<0.01)respectively.This effect was associated with ultrastructural modification of the cyst in ABZ+IFN-αgroup.Interestingly,significant decrease of IL(interleukin)-10 in serum and in vitro production by spleen cells with ABZ+IFN-αtreatment was observed in comparison with untreated control(P<0.01).Serum IgE,IgG and subsets were respectively decreased in ABZ+IFN-αtreatment,compared with that in control group(P<0.01).Our findings demonstrated that combination of ABZ with IFN-αmay contribute to an efficient therapeutic regimen of human and animal CE.

关 键 词：细粒棘球蚴病(CE) 阿苯达唑 IFN-Α IL-10 超微结构改变 

分 类 号：R383[医药卫生—医学寄生虫学]