动物源沙门菌的耐药表型、基因型及流行特征研究  

Epidemiologic characteristics and antibiotic resistance phenotypes and genotypes of Salmonella from food animals

作　　者：岂晓鑫 陈建晧[1] 张小荣 曹永忠[1] 巢国祥[3] 吴艳涛 QI Xiao-xin;CHEN Jian-hao;ZHANG Xiao-rong;CAO Yong-zhong;CHAO Guo-xiang;WU Yan-tao(College of Veterinary Medicine,Yangzhou University,Yangzhou 225002,China;China Institute of Veterinary Drug Control,Beijing 100081,China;Yangzhou Center for Disease Control and Prevention,Yangzhou 225001,China)

机构地区：[1]扬州大学兽医学院,扬州225002 [2]中国兽医药品监察所,北京100081 [3]扬州市疾病预防控制中心,扬州225001

出　　处：《中国人兽共患病学报》2018年第2期150-157,164,共9页Chinese Journal of Zoonoses

基　　金：国家重点研发计划项目(No.2016YFD0501600)资助~~

摘　　要：目的研究动物源沙门菌耐药及流行特征。方法对116株动物源沙门菌进行血清学分型、药物敏感试验、耐药基因(簇)检测和多位点序列(MLST)分析。结果鸡源沙门菌多为ST11克隆肠炎沙门菌和ST17克隆印第安那沙门菌,猪源沙门菌多属于ST40克隆德尔卑沙门菌。ST11克隆主要表现为对氨苄青霉素、萘啶酸、四环素、头孢哌酮耐药,且多数为多重耐药(MDR);ST17克隆多数对9种以上的药物特别是头孢类及氟喹诺酮类耐药,为超级耐药(Super-MDR)克隆。ST11克隆中blaTEM-1-like携带率最高,ST17克隆中blaOXA-1-like、blaCTX-M、blaTEM-1-like及floR、aadA2、sul1及aac(6′)-1b高携带率是其成为超级耐药克隆的主要原因。结论抗菌药物特别是头孢类、氟喹诺酮类药物在动物生产中应当控制使用,对控制耐药菌的产生及传播有重要意义。To study the transmission of Salmonella and resistance genes,116 Salmonella isolates were tested the serotypes,antimicrobial susceptibility,resistant genes on SG1 includingβ-lactamase genes,and multilocus sequence typing(MLST).Results showed that Salmonella isolates from the chicken belonged to ST11 clone,S.enteritidis,and ST17 clone,S.indiana,and from the pig belonged to ST40,S.derby mostly.ST11 clone showed multidrug-resistant(MDR),resistance to ampicillin,nalidixic acid,tetracycline,and cefoperazone,mostly.ST17 clone showed resistance to nine or more kinds of antibiotics including cephalosporins and fluouoquinolones,a super-MDR clone.ST11 clone carried bla TEM-1-like highly,whereas bla OXA-1-like,bla CTX-M,bla TEM-1-like,and floR,aadA2,sul1,and aac(6′)-1b were highly carried in ST17 clone,a new super-MDR clone.The antibiotic abuse and misuse in food supply chains were the main origin of MDR and super-MDR Salmonella,which were transmitted by the chains.It is significance that the control of substance abuse,especially cephalosporins and fluouoquinolones,in food supply chains.

关 键 词：沙门菌 多位点序列分型(MLST) 多重耐药 Β-内酰胺酶基因 

分 类 号：R378[医药卫生—病原生物学]