杂交兰‘黄金小神童'四倍体诱导技术研究  

Tetraploid Induction Technology of Cymbidium Golden Elf 'Sundust'

作　　者：李涵[1] 李慧敏[1] 陆琳[1] 杨少杰 瞿素萍[1] 田敏[1] 黎霞[1] 曹桦[1] Li Han;Li Huimin;Lu Lin;Yang Shaojie;Qu Suping;Tian Min;Li Xia;Cao Hua(National Engineering Research Center for Ornamental Horticulture,Flower Research Institute,Yunnan Academy of Agricultural Sciences,Kunming Yunnan 650100;China;Yunnan Provincial Academy of Science and Technology,Kunming Yunnan 650100,China)

机构地区：[1]云南省农业科学院花卉研究所,国家观赏园艺工程技术研究中心,云南昆明650100 [2]云南省科学技术院,云南昆明650100

出　　处：《西南林业大学学报（自然科学）》2018年第2期70-75,共6页Journal of Southwest Forestry University：Natural Sciences

基　　金：云南省科技厅重点新产品开发计划(2014BB009)资助.

摘　　要：以杂交兰‘黄金小神童'根状茎为材料,采用安磺灵和EMS(甲基磺酸乙酯)为诱导剂进行多倍体材料诱导,分别用安磺灵浓度为0、0.001%、0.002%、0.003%对其根状茎浸泡处理24、48、72 h后,接种到含有EMS浓度为0、20、50、70 mg/L的固体培养基中继续诱导,培养1个月后转接到无EMS的分化培养基上培养,经筛选及植株稳定后获得四倍体植株。结果表明:以安磺灵浓度为0.002%处理48 h,EMS浓度为50 mg/L加入培养基培养,变异效果最佳,变异率为52%,死亡率仅为6%。筛选出的四倍体株系经稳定后,在形态上表现出植株粗壮,叶片增厚、带革质、叶色深绿、叶片扭曲等特征;经气孔显微观察,气孔单位面积显著增大;经染色体数目鉴定,二倍体株系染色体数目为2n=2x=40,四倍体株系染色体数目为2n=4x=80,较二倍体加倍。Using oryzalin and EMS(ethyl methane sulfonate)as the agents to do the polyploidy breeding on the Cymbidium Golden Elf'Sundustr.Rhizomes were treated with oryzalin at concentration of 0,0.001%,0.002%,0.003%for 24,48 and 72 h.After treatment,rhizomes were inoculated to contain a concentration of EMS 0,20,50,70 mg/L solid medium,cultured 1 month transferred to a new culture differentiation medium to obtain tetraploid plants.After selection and plant stabilization,tetraploid plants were obtained.The results showed the concentration of oryzalin is 0.002%processing 48 h,the concentration of EMS is 50 mg/L culture medium was added,the mutation rate is reached 52%,the mortality rate was only 6%.Tetraploid material in the form of plants shown stronger,leaves thicker and leathery,dark green leaves,twisted;porosity increases significantly;chromosome number of diploid strain was 2n=2*=40,chromosome number of tetraploid strain was 2n=4*=80.The chromosomes number of tetraploid strain was 2 times that of the diploid strain.

关 键 词：杂交兰 四倍体 安磺灵 诱导 甲基黄酸乙酯 

分 类 号：S682.31[农业科学—观赏园艺] S335.1[农业科学—园艺学]