愈伤组织和培养温度对花生茎线虫繁殖力的影响  

Effect of callus and culture temperature on reproductive ability of Ditylenchus arachis

作　　者：章淑玲[1] 林谷园[2] 肖顺[3] ZHANG Shuling;LIN Guyuan;XIAO Shun(Department of Horticulture and Garden,Fujian Vocational College of Agriculture,Fuzhou,Fujian 350119,China;Fujian Entiy-Exit Inspection and Quarantine Bureau,Fuzhou,Fujian 350001,China;College of Plant Protection,Fujian Agriculture and Forestiy University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农业职业技术学院园艺园林系,福建福州350119 [2]福建出入境检验检疫局,福建福州350001 [3]福建农林大学植物保护学院,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2018年第2期144-147,共4页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省教育厅中青年项目(JA15759);国家自然科学基金(31201495);福建农业职业技术学院2016年重点课题(2016JS006)

摘　　要：测定了不同的愈伤组织(胡萝卜、甘薯)及培养温度(15、20、25、30、35℃)下花生茎线虫的繁殖力.结果表明:花生茎线虫在胡萝卜和甘薯的愈伤组织上均能繁殖并完成生活史,但在胡萝卜愈伤组织上的繁殖力更强;花生茎线虫在胡萝卜愈伤组织上的适宜培养温度为20~30℃,当温度低于15℃或高于35℃时线虫的繁殖受到抑制;花生茎线虫6个不同地理种群在胡萝卜愈伤组织上的繁殖力没有明显差异.Under different calluses(carrot and sweet potato)and culture temperatures(15,20,25,30,35℃),the reproductive ability of Ditylenchus arachis were evaluated.The results showed that D.arachis could reproduce and complete its life cycle on both carrot callus and Sweet potato callus,but the reproductive ability on carrot callus was higher than that on Sweet potato callus.The optimum temperatures of D.arachis on carrot callus were from 20℃to 30℃,and its reproduction was restrained at temperatures lower than 15℃or higher than 35℃.The reproductive abilities of 6 geographical populations of D.arachis on carrot callus were not signif icantly different.

关 键 词：花生茎线虫 愈伤组织 温度 种群 繁殖力 

分 类 号：S432.4[农业科学—植物病理学]