干旱胁迫下复苏植物牛耳草基因组DNA甲基化分析  

Analysis of DNA methylation of Boea hygrometrica under dehydration stress based on methylation sensitive amplification polymorphism

作　　者：刘杰[1,2] 张潇雅 邓馨[2] LIU Jie;ZHANG Xiaoya;DENG Xin(Laboratory of Protected Horticulture,Jia Sixie Agronomy College,Weifang University of Science and Technology,Shouguang 262700,China;Key Laboratory of Northern Plant Resources,Institute of Botany,the Chinese Academy of Sciences,Beijing 100093,China)

机构地区：[1]潍坊科技学院贾思勰农学院设施园艺实验室,山东寿光262700 [2]中国科学院植物研究所北方资源植物重点实验室,北京100093

出　　处：《福建农林大学学报（自然科学版）》2018年第2期205-211,共7页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：中国科学院植物研究所北方资源植物重点实验室开放课题项目(KLPR2015-01)

摘　　要：利用甲基化敏感扩增多态性(methylation sensitive amplified polymorphism,MSAP)技术,研究干旱复苏过程中,牛耳草叶片DNA甲基化水平及动态变化情况.5对引物对共扩增出376个CCGG位点,其中188个位点发生了甲基化或去甲基化变化.处理组总甲基化水平均高于对照组,且均以Ⅰ型CHG(半甲基化)位点为主,占约32.3%~40.2%.牛耳草干旱复苏过程DNA甲基化和去甲基化同时发生,但以去甲基化为主.此外,qRT-PCR技术检测Bh Dnmt2、Bh CMT2等DNA甲基化相关酶基因在干旱复苏过程中的表达,发现均有明显变化.综上,DNA甲基化参与了牛耳草的抗旱复苏过程,可能是通过甲基化/去甲基化调节基因表达,从而参与牛耳草的抗旱分子反应.The objective of this study was to get an overview of the cytosine methylation dynamics in the resurrection plant Boea hygrometrica during dehydration and rehydration. Methylation sensitive amplified polymorphism( MSAP) method was employed to analyze the levels and change patterns of DNA methylation at different time points( fresh,soil-dried for 5 days,and 14 days,Acclimated after rehydration). A total of 376 CCGG sites were detected by MSAP using 5 primer pairs. MSAP results showed the methylation levels of drought-stress groups were higher than control,mainly in hemimethylation pattern. Methylation and demethylation happened simultaneously during dehydration and rehydration,with altered ratios. Besides,the data of qRT-PCR revealed significant changes in the expression of DNA methylation related genes. In summary,our results provide the first experimental evidence for active roles of DNA methylation and demethylation in dehydration tolerance of B.hygrometrica,probably by regulating gene expression.

关 键 词：牛耳草 干旱胁迫 DNA甲基化 MSAP 

分 类 号：Q945.78[生物学—植物学]