液晶传感竞争免疫法检测天蚕素B  

Label-free liquid crystal immunosensor for detection of cecropin B

作　　者：苏秀霞[1] 张姣[1] 栾崇林[2] 霍文静 徐佳 SU Xiu-xia;ZHANG Jiao;LUAN Chong-lin;HUO Wen-jing;XU Jia(College of Chemistry and Chemical Engineering,Key Laboratory of Auxiliary Chemistry&Technology for Chemical Industry,Ministry of Education,Shaanxi University of Science&Technology,Xi′an 710021,China;Applied Chemistry and Biotechnology Institute,Shenzhen Polytechnic,Shenzhen 518088,China)

机构地区：[1]陕西科技大学化学与化工学院教育部轻化工助剂化学与技术重点实验室,陕西西安710021 [2]深圳职业技术学院应用化学与生物技术学院,广东深圳518088

出　　处：《陕西科技大学学报》2018年第2期74-79,共6页Journal of Shaanxi University of Science & Technology

基　　金：国家自然科学基金项目(21175037);广东省自然科学基金项目(2016A030313847)

摘　　要：制备了一种基于液晶取向变化的非标记液晶型免疫传感器,并结合竞争免疫反应原理,将其用于天蚕素B检测.首先采用硅烷化试剂3-氨丙基三乙氧基硅烷/二甲基十八烷基[3-(三甲氧基硅基)丙基]氯化铵(APTES/DMOAP)混合自组装构建可诱导液晶分子呈均一垂直排列的基底敏感膜,再通过戊二醛交联法将天蚕素B固定到基底表面,当天蚕素B抗体与天蚕素B特异性结合后扰乱了液晶分子的取向,使液晶膜的颜色和亮度发生变化,并通过计算偏光显微镜成像灰度强度,可在0.01~0.1ng/mL范围内定量化分析天蚕素B浓度.结果表明,固定化天蚕素B浓度为150ng/mL,天蚕素B抗体浓度为500ng/mL时,天蚕素B检测限可达0.01ng/mL.本方法具有灵敏度高、特异性好、非标记和操作简单等优点.A label-free liquid crystal immunosensor based on orientation changes of liquid crystals was prepared for the detection of cecropin B through competitive immunization assay.The mixed APTES/DMOAP self-assembled monolayers formed by both long and short alkyls could cause uniform vertical alignment of liquid crystals.The homeotropic-to-tilted transition of liquid crystals induced by the specific binding event between anti-cecropin B antibody and cecropin B immobilized via a cross-linker glutaradehyde could result in the corresponding changes of the color and brightness of liquid crystal film.The gray-scale intensities of optical images of liquid crystal cells were measured to quantitatively analyse cecropin B concentrations at the range of 0.01~0.1 ng/mL and as a result,the limit of detection is as low as 0.01 ng/mL with immobilized cecropin B concentration of 150 ng/mL and anti-cecropin B antibody concentration of 500 ng/mL.This study offers a simple,highly sensitive and specific,lable-free method for cecropin B detection.

关 键 词：液晶 自组装膜 免疫传感器 天蚕素B 天蚕素B抗体 

分 类 号：O656.3[理学—分析化学]