豆粕粒度及容器堆料对地衣芽孢杆菌实验室固态发酵效果的影响  

Effects of Particle Size and Contained Windrow of Soybean Meal on the Laboratory Solid Fermentation with Bacillus licheniformis

作　　者：王若愚 赵若恒 宋增磊 宋晓玲[2] 黄倢 WANG Ruo-yu;ZHAO Ruo-heng;SONG Zeng-lei(College of Fisheries and Life Science,Dalian Ocean University,Dalian,Liaoning 116023;Laboratory for Marine Fisheries Science and Food Production Processes,Qingdao National Laboratory for Marine Science and Technology,Key Laboratory of Maricultural Organism Disease Control of Ministry of Agriculture,Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity,Yellow Sea Fish;College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306)

机构地区：[1]大连海洋大学水产与生命学院,辽宁大连116023 [2]中国水产科学研究院黄海水产研究所,青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室,农业部海水养殖病害防治重点实验室,青岛市海水养殖流行病学与生物安保重点实验室,山东青岛266071 [3]上海海洋大学水产与生命学院,上海201306

出　　处：《安徽农业科学》2018年第10期82-84,88,共4页Journal of Anhui Agricultural Sciences

基　　金：中国-东盟海上合作基金项目(2016-2018);现代农业产业技术体系项目(CARS-47);青岛海洋科学与技术国家实验室鳌山科技创新项目(2015ASKJ02);948计划项目(2016-X56)

摘　　要：[目的]对地衣芽孢杆菌的豆粕固态发酵实验室小试工艺中豆粕的粒度和容器内堆料条件进行优化。[方法]在建立豆粕内活菌的有效计数方法的基础上,分析不同混匀方式对发酵豆粕中菌量计数的影响,并研究不同发酵容器、豆粕粒径和堆料厚度对发酵豆粕菌浓度的影响。[结果]发酵后豆粕在PBS振荡1 min能最大程度地释放其中的细菌,以便后续计数;以4层报纸封口的50 m L离心管为发酵容器,采用经80目筛孔的豆粕颗粒和1.5 cm堆料厚度能获得最优的实验室小试发酵效果,菌浓度能达到1.65×1010~1.76×1010CFU/g。[结论]研究为发酵豆粕实验室小试方法提供了技术支持。[Objective]To optimize the particle size and contained windrow conditions of soybean meal in solid fermentation laboratory trail with Bacillus licheniformis.[Method]Based on establishing the effective counting method of live bacteria in soybean,the effects of different blending methods on the bacteria count in fermented soybean meal were analyzed and the effects of different fermentation vessel,soybean meal’s particle size and windrow thickness on the bacteria concentration of fermented soybean meal were studied.[Result]After fermented soybean meal was oscillated 1 min in PBS,the bacteria were released from the soybean meal for the follow-up counting.Taking 4-layer newspaper-closed 50 mL centrifuge tube as fermentation vessel,when soybean meal particles through an 80 mesh sieve and a windrow with 1.5 cm thickness were used,the best fermentation effect of laboratory trail was obtained and bacteria concentration reached 1.65×10 10~1.76×10 10 CFU/g.[Conclusion]This study could provide technical support for the laboratory trail method of fermented soybean meal.

关 键 词：地衣芽孢杆菌 固态发酵 豆粕 实验室小试 

分 类 号：S816.7[农业科学—饲料科学]