In vitro anther culture and Agrobacterium-mediated transformation of the AP1 gene from Salix integra Linn. in haploid poplar(Populus simonii × P. nigra)  

作　　者：Jingli Yang Kun Li Chunyan Li Junxiu Li Bo Zhao Wei Zheng Yuchi Gao Chenghao Li 

机构地区：[1]State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University [2]Department of Pharmacy,Heilongjiang University of Chinese Medicine [3]Research Center on Life Sciences and Environmental Sciences,Harbin University of Commerce [4]Annoroad Gene Technology Co.,Ltd

出　　处：《Journal of Forestry Research》2018年第2期320-329,共10页林业研究（英文版）

基　　金：supported by The Fundamental Research Funds for the Central Universities(2572015EA01);the Innovation Project of State Key Laboratory of Tree Genetics and Breeding(Northeast Forestry University;grant number 2013A04);Natural Science Fund of Heilongjiang Province(No.QC2015035)

摘　　要：A reliable,efficient anther culture system,the dominant technique for generating haploid plants in breeding programs,that can be used for generating transgenic poplar plants has been needed.In the present study,therefore,an anther culture system was developed using isolated mid-and late-uninucleate anthers of poplar(Populus simonii x P.nigra).From a combination of SSR and ploidy analyses,six double haploid and two haploid lines were characterized from 86 plants grown from 16 regenerated anther cultured lines.After 48 months of development,two plant lines from the regenerated plants maintained their haploid level in vitro for over 2 years.A number of haploid plants from the different lines weretransferred to soil.The leaves of these transplants were then used as explants for transformation with the APETALA1(AP1) gene using Agrobacterium tumefaciens.Overexpression of AP1 in haploid poplar induced early flowering with obvious petals when ectopically expressed.To our knowledge,this is the first report on changes in flowering time in AP1-trangenic poplar,which is important for elucidating the regulatory mechanism of tree flower development.A reliable,efficient anther culture system,the dominant technique for generating haploid plants in breeding programs,that can be used for generating transgenic poplar plants has been needed.In the present study,therefore,an anther culture system was developed using isolated mid-and late-uninucleate anthers of poplar(Populus simonii x P.nigra).From a combination of SSR and ploidy analyses,six double haploid and two haploid lines were characterized from 86 plants grown from 16 regenerated anther cultured lines.After 48 months of development,two plant lines from the regenerated plants maintained their haploid level in vitro for over 2 years.A number of haploid plants from the different lines weretransferred to soil.The leaves of these transplants were then used as explants for transformation with the APETALA1(AP1) gene using Agrobacterium tumefaciens.Overexpression of AP1 in haploid poplar induced early flowering with obvious petals when ectopically expressed.To our knowledge,this is the first report on changes in flowering time in AP1-trangenic poplar,which is important for elucidating the regulatory mechanism of tree flower development.

关 键 词：Pollen grain POPULUS Flower development HAPLOID APETALA1 

分 类 号：S792.11[农业科学—林木遗传育种]