大鼠心脏能量代谢底物随年龄增长的变化及调节  

Changes and Regulation of Cardiac Energy Metabolism Substrates with Age in Rats

作　　者：张霞[1,2] 马丽曼 王妍[4] 刘春[4] 翟艳杰[1] 邢艳秋 ZHANG Xia;MA Liman;WANG Yan(Qilu Hospital of Shandong University,Ji’nan 250012,China)

机构地区：[1]山东大学齐鲁医院,山东济南250012 [2]山东省日照市东港区人民医院 [3]山东省医学科学院附属医院 [4]山东省医学科学院

出　　处：《中国医学创新》2017年第33期23-26,共4页Medical Innovation of China

基　　金：国家自然基金(81570356);山东省科技发展计划(2014GGH218025);山东省医药卫生科技发展计划(2013WS0232)

摘　　要：目的:研究大鼠心肌能量代谢的底物随年龄的变化及调节机制。方法:选用2个月和14个月的雄性SD大鼠,分别为青年对照(young&control,YC)组和老龄对照(aging&control,AC)组。实验末期利用超声心动图测量左心室收缩功能及舒张功能;利用高压液相波谱分析(HPLC)测定心脏ATP的含量;应用Western blot方法检测心肌GLUT-4及CPT-1的表达;用临床生化法检测血Glu、TG、TC、LDL-C、HDL-C、AST、ALT、ALP、BUN、Cr、LDH等指标。结果:实验末期,两组大鼠LVEF比较,差异无统计学意义(P>0.05)。AC组的大鼠心尖ATP含量明显低于YC组,差异有统计学意义(P<0.05)。Western blot检测显示,AC组大鼠GLUT-4与CPT-1表达量较YC组均下降,GLUT-4/CPT-1比值增大(P<0.05)。YC组大鼠血清ALP水平显著高于AC组,差异有统计学意义(P<0.05);两组大鼠的血清TG、Glu、TC、LDL-C、HDL-C、ALT、AST、Cr、BUN以及LD比较,差异均无统计学意义(P>0.05)。结论:大鼠随年龄增长出现的心肌能量代谢改变,能量产生的减少早于心脏功能的改变。Objective:To observe the changes and mechanisms of energy metabolism in the aging rat heart.Method:2 months and 14 months male SD rats were chosen and divided into the young&control(YC)group and the aging&control(AC)group.At the end of the experiment using echocardiographic measurements of left ventricular systolic function and diastolic function;phase spectrum analysis using high pressure liquid(HPLC)determination of cardiac ATP expression;application of Western blot method for detection of myocardial GLUT-4 and CPT-1;by clinical biochemical assay of blood Glu,TG,TC,LDL-C,HDL-C,AST,ALT,ALP,BUN,Cr,LDH and other indicators.Result:At the end of the experiment,there was no significant difference in LVEF between the two groups(P>0.05).The content of apical ATP in the AC group was significantly lower than that in the YC group,the difference was statistically significant(P<0.05).Western blot analysis showed that the expression of GLUT-4 and CPT-1 in AC group were lower than those in YC group,the ratio of GLUT-4/CPT-1 was increased(P<0.05).The serum ALP level of YC group was significantly higher than that of AC group,the difference was statistically significant(P<0.05);the serum TG,Glu,TC,LDL-C,HDL-C,ALT,AST,Cr,BUN and LD in the two groups were not significantly different(P>0.05).Conclusion:The change of myocardial energy metabolism in rats increases with age,and the decrease of energy production is earlier than the change of cardiac function.

关 键 词：老龄化 ATP 葡萄糖转运子-4 肉碱脂酰转移酶-1 

分 类 号：R54[医药卫生—心血管疾病]