绿豆环氧水解酶交联聚集体(CLEAs)的制备  

Preparation of Cross-linked Aggregate of Epoxide Enzyme from Mung Bean

作　　者：贺雪珊 张艳梅 张祥 陈晓莹 刘保全 He Xueshan;Zhang Yanmei;Zhang Xiang;Chen Xiaoying;Liu Baoquan(College of Life Science,Dalian Minzu University,Dalian 116600,China)

机构地区：[1]大连民族大学生命科学学院,辽宁大连116600

出　　处：《山东化工》2018年第3期19-20,共2页Shandong Chemical Industry

基　　金：大连民族大学创新创业训练计划(项目编号:201712026207;201712026425)

摘　　要：从绿豆中提取了环氧水解酶,考察了沉淀方法对酶聚集体活性的影响。结果表明,采用50%饱和度的硫酸铵作为沉淀剂可以得到酶活性保持的酶聚集体;又考察了戊二醛浓度对绿豆环氧水解酶酶交联聚集体活性的影响。结果表明,采用0.4%的戊二醛,酶交联聚集体保持较高的活性,并且可以重复使用。Epoxide enzyme from mung bean was prepared firstly.Then its aggregates were produced using several precipitants.Results indicated that 50%ammonia sulfate was the best precipitant which gave high activity retention.Then,glutaraldehyde solutions with various concentrations were tested as cross-linkers.Results showed that 0.4%glutaraldehyde solution gave the best result of the cross-linked enzyme aggregates,which not only showed high activity but also could be reused.

关 键 词：环氧水解酶 酶聚集体 硫酸铵 戊二醛 交联 

分 类 号：Q814[生物学—生物工程]