天麻素对脊髓损伤模型大鼠神经功能恢复和胶质纤维酸性蛋白表达的影响  

Gastrodin effects on the neural functional recovery and expression of glial fibrillary acidic protein in a rat model of spinal cord injury

作　　者：肖雪飞[1,2] 李娟娟 黄辉 张翔[1] 黄鹞[1] 李经辉[1] 余化霖[1] Xiao Xue-fei;Li Juan-juan;Huang Hui;Zhang Xiang;Huang Yao;Li Jing-hui;Yu Hua-lin(Second Department of Neurosurgery,First Affiliated Hospital of Kunming Medical University,Kunming 650032,Yunnan Province,China;;Department of Histology and Embryology,School of Basic Medicine,Kunming Medical University,Kunming 650500,Yunnan Province,China;Bishan Hospital,the First Affiliated Hospital of Chongqing Medical University,Chongqing 402760,China)

机构地区：[1]昆明医科大学第一附属医院神经外二科,云南省昆明市650032 [2]重庆医科大学第一附属医院璧山医院,重庆市402760 [3]昆明医科大学基础医学院组胚教研室,云南省昆明市650500

出　　处：《中国组织工程研究》2018年第4期558-563,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81560206)；云南省应用基础研究项目(2017FE467-033)；云南省教育厅科学研究基金项目(2013Y287)

摘　　要：背景:研究表明,天麻素在体外可以抑制无血清损伤后星形胶质细胞活化,改善脊髓损伤微环境,减轻继发损伤。目的:观察天麻素对钳夹型大鼠脊髓损伤模型神经功能恢复、胶质纤维酸性蛋白的影响。方法:48只成年SD大鼠随机分为3组,脊髓损伤组建立钳夹型大鼠脊髓损伤模型;假手术组仅切除椎板;天麻素组造模后给予天麻素100 mg/(kg·d)治疗;假手术组和脊髓损伤组给予等量生理盐水。术后分别于1,3,7,14,21,28 d采用BBB运动功能评分和Rivlin斜板实验评估脊髓功能恢复情况,术后28 d进行灌注、取材,观察损伤节段脊髓形态变化、免疫荧光观察胶质纤维酸性蛋白表达变化、尼氏染色观察尼氏小体变化情况。结果与结论:(1)造模后各组大鼠BBB评分和Rivlin斜板实验评分均下降,随着时间推移各组评分逐渐升高,假手术组评分于各时相点均高于其余各组(P<0.05),天麻素组在术后3 d及其后各时相点评分均明显均高于脊髓损伤组(P<0.05);(2)术后28 d,天麻素组和脊髓损伤组胶质纤维酸性蛋白表达较假手术组均明显增加(P<0.05),天麻素组胶质纤维酸性蛋白表达较脊髓损伤组明显降低(P<0.05);(3)天麻素组和脊髓损伤组Nissl小体计数均较假手术组少(P<0.05),染色较浅,部分Nissl小体溶解,天麻素组Nissl小体计数较脊髓损伤组明显增多(P<0.05);(4)假手术组脊髓组织结构完整,脊髓损伤组脊髓结构形态紊乱,脊髓空洞形成,灰白质界限不清,天麻素组脊髓空洞较小,灰白质界限尚清晰;(5)结果表明,天麻素可以在脊髓损伤后抑制星形胶质细胞活化增生和保护神经元,改善肢体运动功能。BACKGROUND:Gastrodin has been shown to inhibit the in vivo astrocyte activation after injury,improve the microenvironment of spinal cord injury(SCI),and reduce secondary injuries.OBJECTIVE:To observe the effects of gastrodine on the neural functional recovery and glial fibrillary acidic protein in a rat model of clamping-induced SCI.METHODS:Forty-eight adult Sprague-Dawley rats were randomly divided into three groups,and the models of clamping-induced SCI were established.The rats in sham group were only subjected to laminectomy;gastrodine group was given 100 mg/kg?d gastrodine;the sham and SCI groups were given equal volume of normal saline.The function recovery of spinal cord was evaluated by Basso,Beattie,Bresnahan(BBB)scores,and Rivlin inclined plane experiment at postoperative 1,3,7,14,21 and 28 days.The morphological changes of spinal cord were observed after perfusion at 28 days,expression level of glial fibrillary acidic protein was detected by immunofluorescence,and changes of Nissl body were observed through Nissl staining.RESULTS AND CONCLUSION:BBB and Rivlin inclined plane scores in each group were significantly decreased postoperatively,and the scores then increased with time;the scores at each time point in the sham group were significantly higher than those in the other groups(P<0.05);the scores in the gastrodine group were significantly higher than those in the SCI group from day 3(P<0.05).At postoperative 28 days,the expression level of glial fibrillary acidic protein was the lowest in the sham group,followed by gastrodine group,and the highest in the SCI group(P<0.05).In the gastrodine and SCI groups,Nissl bodies were slightly stained partially dissolved,and the account of Nissl bodies in this two groups was less than that in the sham group(P<0.05),but the gastrodine group had significantly more Nissl bodies than the SCI group(P<0.05).The spinal cord in the sham group revealed the complete structure;in the SCI group,the structure of spinal cord was in disorder,syringomyelia formatted,and the gra

关 键 词：天麻素 神经损伤与修复动物模型 钳夹型脊髓损伤 运动功能 星形胶质细胞 神经元 尼氏小体 运动功能 组织构建 

分 类 号：R318[医药卫生—生物医学工程]