组织工程心脏:电生理特性及长期安全性?  

Tissue-engineered heart: electrophysiological properties and long-term safety

作　　者：仝彩玲 李鸣晖 齐忠权 Tong Cai-ling;Li Ming-hui;Qi Zhong-quan(School of Life Science Xiamen University,Xiamen 361102,Fujian Province,China;Organ Transplantation Institute,Xiamen University,Xiamen 361102,Fujian Province,China;the Key Laboratory of Organ and Tissue Regeneration of Fujian Province,Xiamen 361102,Fujian Province,China)

机构地区：[1]厦门大学生命科学学院,福建省厦门市361102 [2]厦门大学器官移植研究所,福建省厦门市361102 [3]福建省器官与组织再生重点实验室,福建省厦门市361102

出　　处：《中国组织工程研究》2018年第4期606-611,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:由于供体心脏严重短缺并受制于伦理道德,很多需要心脏移植的患者最终因缺乏供体心脏而死亡。理论上组织工程心脏是解决供体心脏不足的重要手段。目的:回顾分析组织工程心脏构建用的支架材料、种子细胞及细胞接种培养方法,为后期组织工程心脏的构建做参考。方法:由作者检索2004年至2016年Pub Med数据库及SCI(web of science)数据库关于组织工程心脏构建方法研究的文献,共检索文献2 921篇,按照纳入和排除标准进行筛选,共纳入53篇。结果与结论:器官的体外培养及组织工程心脏功能是组织工程心脏构建的难点。组织工程心脏体外培养需要提供心肌细胞增殖需要的营养、气体、温度及相应的电刺激。心肌细胞的诱导、支架材料的获取及体外培养系统的建立是组织工程心脏构建过程中不可缺少的条件。优化支架材料的脱细胞流程,整合理想的种子细胞并提高其在支架内的黏附、增殖和分化能力,通过基因调控改善组织工程心脏的电生理特性,长期的安全性分析等,每一项研究目标都任重而道远。BACKGROUND:Insufficiency of donor heart and ethics are the major obstacles to heart transplantation.Theoretically,a tissue-engineered heart is an important means to solve the donor heart insufficiency.OBJECTIVE:To review the scaffold materials,seed cells and cell incubation methods in the construction of tissue-engineered heart,thus providing references for the future study on the tissue-engineered heart.METHODS:A retrieval of PubMed and Web of Science databases was performed for the articles addressing the construction of tissue-engineered heart from 2004 to 2016.Totally 2 921 articles were searched,and finally 53 eligible articles were included in accordance with the inclusion and exclusion criteria.RESULTS AND CONCLUSION:In vitro organ culture and the function of tissue-engineered heart are the difficulties in the construction of tissue-engineered heart.In vitro construction of tissue-engineered heart requires the supply of nutrients,gases,temperature and corresponding electrical stimulation.Myocardial cells,scaffold materials and organ culture system are indispensable for the tissue-engineered heart construction.Therefore,it is highly important to optimize the decellular process,select an ideal seed cell and improve its adhesion,proliferation and differentiation,improve the electrophysiological properties of the tissue-engineered heart by gene regulation,and confirm the long-term safety of the tissue-engineered heart.

关 键 词：组织工程心脏 支架材料 去细胞方法 组织构建 细胞接种 

分 类 号：R318[医药卫生—生物医学工程]