宫颈病变内高危型HPV感染与Th细胞分化、细胞异常增殖的相关性  

The correlation of high-risk HPV infection in cervical lesions with Th cell differentiation and abnormal cell proliferation

作　　者：刘琳[1] 沈攀 张力忆[3] LIU Lin;SHEN Pan;ZHANG Li yi(Department of Pathology,Dazhou Central Hospital in Sichuan Province,Dazhou,Sichuan Province,635000;Department of General Surgery,Dazhou Central Hospital in Sichuan Province,Dazhou,Sichuan Province,635000;Department of Obstetrics and Gynecology,Dazhou Central Hospital in Sichuan Province,Dazhou,Sichuan Province,635000)

机构地区：[1]四川省达州市中心医院病理科,四川达州635000 [2]四川省达州市中心医院普外科,四川达州635000 [3]四川省达州市中心医院妇产科,四川达州635000

出　　处：《海南医学院学报》2018年第3期315-318,共4页Journal of Hainan Medical University

基　　金：达州市2017年市级医学科研立项(201701)~~

摘　　要：目的:探讨宫颈病变内高危型HPV感染与Th细胞分化、细胞异常增殖的相关性。方法:选择在本院进行妇科检查并诊断为高危型HPV感染的患者80例、低危型HPV感染的患者64例分别作为高危型HPV感染组、低危型HPV感染组,同期在本院进行检查的健康女性50例作为正常对照组。对比三组研究对象宫颈组织中Th1/Th2细胞因子、Th17/Treg细胞因子及增殖相关基因表达量的差异。结果:高危型HPV感染组宫颈组织中IL-2、IL-3的含量低于低危型HPV感染组、正常对照组,IL-4、IL-10的含量高于低危型HPV感染组、正常对照组;IL-17、IL-23、IL-6、TGF-β的含量均高于低危型HPV感染组、正常对照组;Prdx4、Furin、STAT3 mRNA的表达量高于低危型HPV感染组、正常对照组,FOXF2mRNA的表达量低于低危型HPV感染组、正常对照组。结论:高危型HPV感染可直接影响宫颈组织的Th1/Th2、Th17/Treg细胞平衡,同时促使病灶区域宫颈细胞异常增殖。Objective:To investigate the correlation of high risk HPV infection in cervical lesions with Th cell differentiation and abnormal cell proliferation.Methods:80 patients who underwent gynecological examination and were diagnosed with high risk HPV infection and 64 patients who were diagnosed with low risk HPV infection in our hospital between August 2016 and December 2017 were enrolled in high risk HPV infection group and low risk HPV infection group respectively,and 50 healthy women who underwent examination in our hospital during the same period were enrolled in normal control group.The differences in the expression levels of Th1/Th2 cytokines,Th17/Treg cytokines and proliferation related genes in cervical tissue were compared among the three groups.Results:IL 2 and IL 3 levels in cervical tissue of high risk HPV infection group were lower than those of low risk HPV infection group and normal control group whereas IL 4 and IL 10 contents were higher than those of low risk HPV infection group and normal control group;IL 17,IL 23,IL 6 and TGFβcontents were higher than those of low risk HPV infection group and normal control group;Prdx4,Furin and STAT3 mRNA expression were higher than those of low risk HPV infection group and normal control group whereas FOXF2 mRNA expression was lower than that of low risk HPV infection group and normal control group.Conclusion:High risk HPV infection can directly affect the balance of Th1/Th2 and Th17/Treg cells in cervical tissues,and promote the abnormal proliferation of cervical cells in the lesion area.

关 键 词：宫颈癌 高危型HPV感染 TH1/TH2细胞 TH17/TREG细胞 增殖基因 

分 类 号：R711.74[医药卫生—妇产科学]