ABR-MBR组合工艺短程硝化过程的微生物种群  

Analysis of microbial population of shortcut nitrification in ABR-MBR process

作　　者：赵诗惠 吕亮[1,2] 蒋志云 吴忆宁 吴鹏 沈耀良[1,2,3] ZHAO Shi-hui;LüLiang;JIANG Zhi-yun;WU Yi-ning;WU Peng;SHEN Yao-liang(School of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou Jiangsu 215009,China;Jiangsu Collaborative Innovation Center of Water Treatment Technology and Material,Suzhou Jiangsu 215009,China;Key Laboratory of Environmental Science and Engineering of Jiangsu Province,Suzhou Jiangsu 215009,China)

机构地区：[1]苏州科技大学环境科学与工程学院,江苏苏州215009 [2]江苏省水处理技术与材料协同创新中心,江苏苏州215009 [3]江苏省环境科学与工程重点实验室,江苏苏州215009

出　　处：《中国环境科学》2018年第2期566-573,共8页China Environmental Science

基　　金：国家自然科学基金资助项目(51578353);江苏省自然科学基金资助项目(BK20160356);江苏省高校自然科学研究项目(16KJB610013);苏州科技学院科研基金青年项目(XKQ201504)

摘　　要：采用Miseq高通量测序技术研究氨氮进水负荷对ABR-MBR组合工艺MBR池中微生物种群的丰度及优势菌群的影响.结果表明,温度为28~32℃、p H值为7.1~7.4、DO为0.5~1mg/L并逐步提高氨氮进水负荷的条件下,可以使氨氧化菌(AOB)大量富集,并抑制亚硝酸盐氧化菌(NOB)的活性,从而实现短程硝化的稳定运行.在氨氮进水负荷为0.94kg/(m^3·d)时,平均亚硝酸盐积累率达到60%以上,氨氮去除率稳定在90%.在系统运行过程中,变形菌门是系统中的优势菌门,Nitrosomonas的相对丰度由4.97%升至22.56%,硝化螺菌属的相对丰度为0.06%~2.12%.因此,ABR-MBR组合工艺短程硝化过程中亚硝酸盐积累率与AOB的活性、相对丰度密切相关,即AOB的大量富集可以有效实现短程硝化,而NOB的小幅度增长不会影响短程硝化的实现.系统中微生物种群的多样性和功能微生物的结构稳定性保证了ABR-MBR工艺具有稳定和较好的处理效果.The effects of influent ammonium loading rate(ALR)on the species abundance of microbial communities and dominant bacterial in the ABR-MBR combined process were investigated by Miseq high-throughput sequencing.The results indicated that the numbers of ammonium oxidizing bacteria(AOB)can be significantly increased and the bio-activities of nitrite oxidizing bacteria(NOB)can be inhibited at the temperature of 28~32℃,pH of 7.1~7.4 and DO of 0.5~1mg/L by gradually increasing the influent ALR in the MBR.So that the shortcut nitrification will be achieved efficiently and stably.When influent ALR was 0.94kg/(m3·d),the average nitrite accumulation rate was above 60%,and NH4+-N removal rate was 90%.Proteobacteria were the dominant bacterial.The relative abundance of Nitrosomonas was increased from 4.97%to 22.56%,the relative abundance of Nitrospira was increased from 0.06%to 2.12%during the operation.Therefore,the nitrite accumulation rates were closely related to the bio-activities and abundance of AOB of shortcut nitrification the process.Its showed that shortcut nitrification can be efficiently achieved by a large number of AOB growth.Still,a slight increase of NOB abundance had little effect on the realization of shortcut nitrification.The microbial diversity and its functional structure stability were the guarantee of a stable and high removal efficiency in the ABR-MBR combined process.

关 键 词：短程硝化 氨氧化菌 亚硝酸盐氧化菌 微生物种群 高通量测序 

分 类 号：X703[环境科学与工程—环境工程]