叶酸及不同剂量瑞舒伐他汀对HHcy小鼠动脉粥样硬化相关基因表达的影响  

Influence of differnce doses of rosuvastatin and folic acid on the expression of atherosclerosis related gene for hyperhomocysteinemia mice

作　　者：刘爱宁 杨文刚 鞠树红 孙一鸣 马骏[2] 周晖[3] LIU Ai-ning;YANG Wen-gang;JUShu-hong;SUNYi-ming;MA Jun;ZHOU Hui(Deparment of Neurology,Liqun Hospital of Putuo District,Shanghai 200333,China;Nuclear Medicine Laboratory,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Department of Neurology,Changzheng Hospital,the Second Military Medical University,Shanghai 200003,China)

机构地区：[1]上海市普陀区利群医院神经内科,上海200333 [2]上海中医药大学核医学实验室,上海201203 [3]第二军医大学附属长征医院神经内科,上海200003

出　　处：《河北医科大学学报》2018年第4期378-382,共5页Journal of Hebei Medical University

基　　金：上海市卫生和计划生育委员会科研课题面上项目(201540186)

摘　　要：目的探讨叶酸及不同剂量瑞舒伐他汀对高同型半胱氨酸(hyperhomocysteinemia,HHcy)小鼠动脉粥样硬化(atherosclerosis,AS)相关基因表达的影响。方法将46只小鼠随机分为5组:对照组(n=6)给予普通饲料喂养;高蛋氨酸组(n=10)给予普通饲料喂养+质量分数2%的蛋氨酸;叶酸组(n=10)在高蛋氨酸饲料喂养4周后,给予0.8 mg/kg叶酸灌胃,同时持续高蛋氨酸饮食;瑞舒伐他汀低剂量组(n=10)、高剂量组(n=10)在高蛋氨酸饲料喂养4周后,给予瑞舒伐他汀0.8 mg/kg、1.6 mg/kg灌胃,同时持续高蛋氨酸饮食。12周后采血处死,检测各组小鼠血清Hcy,血脂指标TG、TC、HDL-C、LDL-C水平,炎性指标hs CRP、TNF-α、MMP-9和AS相关基因CXCL1、ICAM-1、VCAM-1表达情况。结果高蛋氨酸组、叶酸组、低剂量组Hcy均高于对照组,叶酸组、低剂量组和高剂量组均低于高蛋氨酸组,高剂量组又低于叶酸组(P<0.05)。高蛋氨酸组、叶酸组、低剂量组TC均高于对照组,低剂量组和高剂量组低于高蛋氨酸组,高剂量组低于叶酸组和低剂量组(P<0.05);高蛋氨酸组TG高于对照组,高剂量组低于高蛋氨酸组(P<0.05);高蛋氨酸组、叶酸组、低剂量组LDL-C高于对照组,叶酸组、低剂量组和高剂量组低于高蛋氨酸组,高剂量组低于叶酸组和低剂量组(P<0.05);高蛋氨酸组、叶酸组、低剂量组HDL-C低于对照组,高剂量组高于高蛋氨酸组、叶酸组和低剂量组(P<0.05)。与对照组比较,高蛋氨酸组、叶酸组、瑞舒伐他汀低剂量组和高剂量组血清hs CRP、TNF-α水平明显升高,MMP-9明显下降(P<0.05);高剂量组各炎性指标均明显低于高蛋氨酸组、叶酸组及低剂量组(P<0.05)。叶酸组、瑞舒伐他汀低剂量组和高剂量组主动脉组织CXCL1、ICAM-1、VCAM-1、MMP-9阳性细胞百分比明显低于高蛋氨酸组(P<0.05);与叶酸组、低剂量组比较,高剂量组各AS相关基因阳性细胞百分比明显降低(P<0.05);高蛋氨酸组、叶酸组�Objective To explore the influence of different doses of rosuvastatin and folic acid on the expression of atherosclerosis(AS)related gene for hyperhomocysteinemia(HHcy)mice.Methods Forty-six mice were randomly divided into five groups.Control group(n=6)was given with ordinary diet.High methionine group(n=10)was given with 2%methionine+ordinary diet,folic acid group(n=10)was given with 0.8 mg/kg folic acid by gavage and 2%methionine after methionine for 4 weeks.Rosuvastatin group of low dose(n=10),high dose(n=10)was given with 0.8 mg/kg,1.6 mg/kg rosuvastatin by gavage and 2%methionine after methionine for 4 weeks.All mice were executed by drawing-out blood after 12 weeks,the serum Hcy,blood lipid indicators including TG,TC,HDL-C,LDL-C,hsCRP,TNF-α,MMP-9,the expression of AS related genes including CXCL1,ICAM-1,VCAM-1 were detected.Results The level of HHcy in high methionine group,folic acid group,low dose group was higher than that of control group,and folic acid group,low dose group and high dose group was lower than high methionine group,high dose group was lower than folic acid group(P<0.05).The level of TC in high methionine group,folic acid group,low dose group was higher than that of control group,and folic acid group,low dose group and high dose group was lower than high methionine group,high dose group was lower than folic acid group and low dose group(P<0.05).The level of TG in high methionine group was higher than that of control group,and high dose group was lower than high methionine group(P<0.05).The level of LDL-C in high methionine group,folic acid group,low dose group was higher than that of control group,and folic acid group,low dose group and high dose group was lower than high methionine group,high dose group was lower than folic acid group and high dose group(P<0.05).The level of HDL-C in high methionine group,folic acid group,low dose group was lower than that of control group,and high dose group was higher than high methionine group,folic acid group and low dose group(P<0.05).Compared with

关 键 词：动脉粥样硬化 瑞舒伐他汀 叶酸 小鼠 

分 类 号：R543.4[医药卫生—心血管疾病]