颗粒细胞骨形态发生蛋白9评估IVF卵母细胞成熟及胚胎发育  

Assessment of IVF oocyte maturation and embryonic development by bone morphogenetic protein-9 of granulosa cells

作　　者：刘灿 汪辉 李怡 张坪[1] 江乾[1] 甄秀丽[1] LIU Can;WANG Hui;LI Yi;ZHANG Ping;JIANG Qian;ZHEN Xiu-li(Department of Reproductive Medical Center,the Forth Hospital of Hebei Medical University,Shijiazhuang 050011,China)

机构地区：[1]河北医科大学第四医院生殖医学科,河北石家庄050011

出　　处：《河北医科大学学报》2018年第4期425-430,共6页Journal of Hebei Medical University

基　　金：河北省科技计划项目(16277774D)

摘　　要：目的检测骨形态发生蛋白9(bone morphogenetic protein-9,BMP-9)在人卵巢颗粒细胞中的表达水平,探讨其与卵母细胞及胚胎质量的关系。方法收集85例因输卵管因素行常规体外受精-胚胎移植(in vitro fertilization and embryo transfer,IVF-ET)患者的颗粒细胞,采用免疫细胞化学法、逆转录PCR和Western blot检测颗粒细胞中BMP-9的表达。结果 BMP-9表达于颗粒细胞胞浆中,呈棕黄色颗粒。颗粒细胞中BMP-9 m RNA表达与蛋白表达呈正相关(r=0.220,P<0.05)。BMP-9 m RNA及蛋白的表达与患者临床数据及实验室相关数据均无直线相关关系。根据BMP-9 m RNA相对表达量的均数将患者分为低表达组和高表达组,高表达组的优质胚胎率、卵子利用率均高于低表达组,差异有统计学意义(P<0.05)。结论 BMP-9表达于人卵巢颗粒细胞胞浆内,BMP-9高表达可提高优质胚胎率,其表达水平在一定程度上可反映卵母细胞的质量。BMP-9可能作为自分泌/旁分泌因子参与生殖系统调控。Objective To detect the expression level of bone morphogenetic protein-9(BMP-9)in human ovarian granulosa cells,and to discuss its relationship to the maturation of oocyte and development of embryo.Methods Eighty-five cases were collected from the patients taking in vitro fertilization(IVF)with fallopian tube factor.The expression of BMP-9 in granulosa cells were measured by immunocytochemistry,RT-PCR and Western blot.Results BMP-9 was expressed in the cytoplasm of granulosa cells.There are positive correlation between BMP-9 mRNA expression and protein expression in granular cells(r=0.220,P<0.05).The expression of BMP-9 mRNA and protein in granulosa cells do not have linear correlation with clinical and laboratory IVF data.According to the mean of relative expression of BMP-9 mRNA,the patients were divided into low expression group and high expression group.High quality embryos and egg utilization in high expression group were significantly higher than those in low expression group(P<0.05).Conclusion BMP-9 expressed in the cytoplasm of human ovarian granulosa cells.High expression of BMP-9 can increase the rate of high quality embryos,and its expression level in a certain extent can reflect the quality of oocytes.BMP-9 may act as an autocrine/paracrine factor involved in the regulation of the reproductive system.

关 键 词：体外受精 胚胎移植 骨形态发生蛋白质9 

分 类 号：R321.3[医药卫生—人体解剖和组织胚胎学]