UHPLC-ELSD检测怀牛膝中的三七皂苷R_1和竹节参皂苷Ⅳa的含量  

Simultaneous determination of Notoginsenoside R_1 and Chikusetsusaponin IVa in Achyranthes bidentata Bl by UHPLC-ELSD

作　　者：戴丽[1] 吴虹[1] 傅俊[1] 邓然[1] 李锋[1] DAI Li;WU Hong;FU Jun;DENG Ran;LI Feng(School of Pharmacy,Anhui University of Chinese Medicine,Anhui Province Key Laboratory of R&D of Chinese Medicine,Anhui Hefei 230012,China)

机构地区：[1]安徽中医药大学安徽省中药研究与开发重点实验室,安徽合肥230012

出　　处：《广州化工》2018年第6期92-94,共3页GuangZhou Chemical Industry

摘　　要：建立超高效液相色谱法(ultra-high performance liquid chromatography-evaporative light-scatting detector,UHPLCELSD)同时测定怀牛膝中三七皂苷R_1和竹节参皂苷IVa的含量。采用Waters ACQUITY ODS C_(18)色谱柱(2.1×50 mm,1.7μm);以乙腈-0.1%甲酸水溶液梯度洗脱,流速0.16 mL/min;漂移管温度105℃;载气流速2.9 L/min。怀牛膝中三七皂苷R_1和竹节参皂苷IVa分别在0.51～1.53 mg、0.62～1.86 mg范围内呈良好的线性关系;平均加样回收率分别为99.90%、100.67%;RSD值分别为4.37%、1.29%。该方法简便、高效、重复性及专属性良好,适用于同时测定怀牛膝中三七皂苷R_1和竹节参皂苷IVa的含量。The study aimed to develop a reversed-phase ultra-high performance liquid chromatography-evaporative light-scatting detector(UHPLC-ELSD)method for simultaneous determination of notoginsenoside R1 and chikusetsusaponin IVa in Radix Achyranthis Bidentatae.Waters ACQUITY ODS C 18(2.1×50 mm,1.7μm)column was used with mobile phase consisted of acetonitrile(A)-water(B)with 0.1%formic acid by gradient elution at a flow rate of 0.160 mL/min.The temperature of drift tube was 105℃,the flow rate of carrier gas was 2.9 L·min-1.Notoginsenoside R1 and chikusetsusaponin IVa in Radix Achyranthis Bidentatae had good linearity within the range of 0.51~1.53 mg and 0.62~1.86 mg,respectively.The average recoveries were 99.90%and 100.67%,and RSD values were 4.37%and 1.29%,respectively.The method is more simple,efficient,has excellent repeatability and specificity for simultaneous determination of notoginsenoside R1 and chikusetsusaponin IVa in Radix Achyranthis Bidentatae.

关 键 词：怀牛膝 三七皂苷R 1 竹节参皂苷IVa UHPLC-ELSD法 含量测定 

分 类 号：R917[医药卫生—药物分析学]