左旋龙脑对人脐静脉内皮细胞氯通道和细胞容积的影响  

Effects of L-borneol on chloride channel and cell volume in human umbilical vein endothelial cells

作　　者：梁协稠 陈湛如 许经逵 封洁珠 伍嘉宝 徐剑英 高宏[2] 谭秋婵 陈丽新[2] 王立伟[1] LIANG Xie-chou;CHEN Zhan-ru;XU Jing-kui;FENG Jie-zhu;WU Jia-bao;XU Jian-ying;GAO Hong;TAN Qiu-chan;CHEN Li-xin;WANG Li-wei(Dept of Physiology,Medical College,Jinan University,Guangzhou　510632,China;Dept of Pharmacology,Medical College,Jinan University,Guangzhou　510632,China;Institute of Birth Control Science and Technology of Guangdong Province,Guangzhou　510600,China;Dept of Clinical Medicine,Medical College,Jinan University,Guangzhou　510632,China)

机构地区：[1]暨南大学医学院生理学系,广东广州510632 [2]暨南大学医学院药理学系,广东广州510632 [3]广东省计划生育科学技术研究所,广东广州510600 [4]暨南大学医学院临床医学系,广东广州510632

出　　处：《中国药理学通报》2018年第4期550-556,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81273539;81272223);广东省科技计划项目(No 2017A050501021);广东省科技计划项目(No 2013B051000059);广东省自然科学基金资助项目(No 2016A030313495)

摘　　要：目的研究左旋龙脑对人脐静脉内皮细胞株(human umbilical vein endothelial cells,HUVECs)氯通道和细胞容积的作用。方法膜片钳技术记录全细胞氯电流;siRNA干扰技术下调ClC-3表达;动态图像分析测量细胞容积。结果 20 nmol·L^(-1)左旋龙脑能明显激活HUVECs氯电流,电流密度达(79.59±4.90)pA/pF,左旋龙脑激活的电流能被氯通道阻断剂NPPB、DIDS抑制,外向电流抑制率分别为(95.57±2.57)%、(97.28±6.36)%;下调ClC-3氯通道表达后,左旋龙脑激活的氯电流明显减小至(27.03±3.89)pA/pF;左旋龙脑能明显诱导细胞容积缩小(14.38±1.58)%,氯通道阻断剂NPPB阻断左旋龙脑诱导的细胞容积改变。结论左旋龙脑能激活HUVECs的ClC-3氯通道,使Cl-外流诱发细胞容积减小。Aim To study the effects of L-borneol on the chloride channel and cell volume of human umbilical vein endothelial cells(HUVECs).Methods Whole-cell patch-clamp technique was used to record chloride currents.The expression of ClC-3 protein was down-regulated by siRNA interference technique.The cell volume was measured by dynamic image analysis.Results 20 nmol·L-1 L-borneol significantly activated chloride current in HUVEC(79.59±4.90)pA/pF,which could be inhibited by chloride channel blockers,NPPB and DIDS.The outward current inhibitory rate of NPPB was(95.57±2.57)%,while that of DIDS was(97.28±6.36)%.The chloride current activated by L-borneol significantly decreased after the silence of ClC-3(27.03±3.89)pA/pF.Cell volume was markedly reduced by L-borneol(14.38±1.58)%,which was inhibited after NPPB appliance.Conclusion L-borneol can activate ClC-3 chloride channel in HUVECs,which induces Cl-outflow then cell volume decrease.

关 键 词：左旋龙脑 人脐静脉内皮细胞 氯通道 膜片钳技术 细胞容积 CLC-3 

分 类 号：R322.123[医药卫生—人体解剖和组织胚胎学] R282.71[医药卫生—基础医学]