消癌解毒方通过调控PI3K/AKT通路抑制乳腺癌细胞株MDA-MB-231增殖的作用与机制探讨  

The Anti-Proliferative Activity and Mechanism Exploration of Xiao'ai Jiedu Decoction on Breast Cancer Cell Lines MDA-MB-231 Through PI3K/AKT Pathway

作　　者：孙浩 沈卫星 徐长亮 孙东东 谭佳妮 许惠琴[1,2] 程海波 SUN Hao;SHENG Wei-xin;XU Chang-liang;SUN Dong-dong;TAN Jia-ni;XU Hui-qin;CHENG Hai-bo(College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing,210023,China;Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica,Nanjing,210023,China;Translational Medicine Research Center,Nanjing University of Chinese Medicine,Key Laboratory of Famous Doctors'Proved Recipe Evaluation and Transformation of State Administration of Traditional Chinese Medicine(TCM),Provincial Lab of Anticarcinoma Proved Recipe Research and Engineering Industrial;TCM Treatment on Tumor Collaborative Innovation Center of Jiangsu Province,Nanjing,210023,China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]江苏省中药药效与安全性评价重点实验室,江苏南京210023 [3]南京中医药大学转化医学研究中心,国家中医药管理局名医验方评价与转化重点研究室,江苏省抗肿瘤验方与产业化工程实验室,江苏南京210023 [4]江苏省中医药防治肿瘤协同创新中心,江苏南京210023

出　　处：《南京中医药大学学报》2018年第2期173-177,共5页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金(81373511,81573910,81673559);国家自然科学基金青年基金(81302829,81403079);江苏省自然科学基金(BK20131416,BK20141467,BK20161045);江苏省高校自然科学研究项目(16KJB360001);江苏高校优势学科建设工程二期资助项目

摘　　要：目的研究消癌解毒方抗乳腺癌细胞的活性及作用机制。方法显微镜观察MDA-MB-231细胞形态,MTT法考察细胞的增殖抑制率,流式细胞术观察细胞周期,Western blot检测PI3K/AKT信号通路相关蛋白P53、P21、Cyclin B1、CDK1的表达。结果与对照组相比,消癌解毒方给药组细胞减少,排列疏松,体积变小,形态变圆;消癌解毒方可以提高MDA-MB-231细胞的增殖抑制率(P<0.05~0.01),使肿瘤细胞的细胞周期阻滞于G2/M期(P<0.05~0.01),可以抑制与细胞周期相关的PI3K/AKT信号通路,上调P53、P21,下调Cyclin B1、CDK1蛋白的表达(P<0.05~0.01)。结论消癌解毒方能阻滞MDA-MB-231细胞周期,抑制乳腺癌细胞的增殖,其机制可能与抑制PI3K/AKT信号通路有关。OBJECTIVE To study the anti proliferative activity and mechanism of Xiao'ai Jiedu decoction against breast cancer cell lines MDA MB 231.METHODS The cell morphology was observed by microscope.MTT method was used to investigate the inhibitory rate of Xiao'ai Jiedu decoction against breast cancer cell lines MDA MB 231.Flow cytometry analyzed the cell cycle distribution.Western blot detected the expression of Cyclin B1 and CDK1 and the expression of upstream signaling molecules P53,P21 in PI3K/AKT signaling pathway.RESULTS Compared with the control group,MDA MB 231 cells of Xiao'ai Jiedu decoction group showed number decreased,loosely arranged,morphology smaller and rounder.Xiao'ai Jiedu decoction inhibited the proliferation of MDA MB 231 cells(P<0.05,P<0.01).Xiao'ai Jiedu decoction down regulated the expression of Cyclin B1 and CDK1,inhibited the PI3K/AKT signaling pathway,and up regulated the expression of P53 and P21(P<0.05,P<0.01).CONCLUSION Xiao'ai Jiedu decoction can inhibit the proliferation of MDA MB 231 breast cancer cells,perhaps related to inhibiting the PI3K/AKT signaling pathway.

关 键 词：消癌解毒方 乳腺癌细胞 细胞增殖 PI3K/AKT通路 

分 类 号：R285.5[医药卫生—中药学]