纳米金刚石拉曼生物探针用于抗菌肽杀菌过程的可视化研究  

Nanodiamonds Raman Probes for Visualizing the Interaction between Antimicrobial Peptide and Bacteria

作　　者：李丹丹 陈鑫 王宏[3] 付杨 余愿 只金芳[1] LI Dandan;CHEN Xin;WANG Hong;FU Yang;YU Yuan;ZHI Jinfang(Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Beijing 100190,P.R.China;University of Chinese Academy of Sciences,Beijing 100049,P.R.China;School of Pharmaceutical Sciences,Peking University,Beijing 100191,P.R.China)

机构地区：[1]中国科学院理化技术研究所,北京100190 [2]中国科学院大学,北京100049 [3]北京大学药学院,北京100191

出　　处：《影像科学与光化学》2018年第1期89-99,共11页Imaging Science and Photochemistry

基　　金：国家自然科学基金项目(21375137);东南大学生物电子国家重点实验室开放基金资助

摘　　要：本工作以纳米金刚石为探针,依赖拉曼成像技术,成功实现了对细菌体系生命活动的观察。实验中将抗菌肽死亡素负载于100nm的金刚石上,利用纳米金刚石在1332cm-1处的特征拉曼信号为标记,通过共聚焦拉曼成像技术可视化了纳米金刚石-死亡素复合物与枯草芽孢杆菌间的相互作用过程。同时,采用扫描电子显微镜观察手段验证了上述拉曼成像方法的有效性。此外,抗菌实验验证了纳米金刚石-死亡素复合物对枯草芽孢杆菌有达到45%的明显杀灭效果,表明纳米金刚石探针的引入不会影响死亡素的抗菌性能。本工作证实了纳米金刚石拉曼生物探针用于观察抗菌过程的可行性,为其在生物成像领域中的应用提供了重要依据。This article realizes the application of nanodiamonds(NDs)Raman probes for vital activity observation in bacteria system.The antimicrobial peptide thanatin was immobilized on 100 nm NDs,and the interaction of the NDs-thanatin conjugate with Bacillus subtilis was visualized via Raman imaging with the intrinsic diamond Raman signal(1332 cm-1)as label.Scanning electron microscope was further utilized to verify the practicability of Raman mapping.Besides,antibacterial activity of NDs-thanatin conjugate was validated,suggesting the adsorbed thanatin on NDs remained superior capability.The results showed the feasibility of nanodiamonds as Raman probes to visualize the bacteria activity,indicating the potential use of NDs in bio-imaging applications.

关 键 词：纳米金刚石 拉曼生物探针 抗菌肽 杀菌活性 可视化 

分 类 号：O657.37[理学—分析化学] TB383.1[理学—化学]