CpG-ODN 2006对斑马鱼抗创伤弧菌感染的作用  

Functions of CpG-ODN 2006 on Resistance of Zebra Fish against Vibrio vulnificus

作　　者：陈华[1] 李素一[1] 张丽娟[1] 柯翎[1] 陈叙[1] 林晨韬[1] CHEN Hua;LI Su-yi;ZHANG Li-juan;KE Ling;CHEN Xu;LIN Chen-tao(Institute of Biotechnology,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian　350003,China)

机构地区：[1]福建省农业科学院生物技术研究所,福建福州350003

出　　处：《福建农业学报》2018年第1期1-6,共6页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划项目--省属公益类科研院所基本科研专项(2015R1019-10;2016R1018-6;2016R1018-3);福建省农业科学院青年人才创新基金项目(2014QB-29);福建省农业科学院科技创新团队PI项目(2016PI-22);福建省农业科学院青年英才计划项目(YC2015-20);福建省自然科学基金项目(2015J01104);国家自然科学基金项目(31100658;31300766)

摘　　要：为创建环境友好的免疫预防技术,分析CpG-ODN对斑马鱼抗创伤弧菌感染的作用,采用PBS(对照)和人工合成的CpG-ODN 2006(试验组)序列分别注射斑马鱼,48h后创伤弧菌FJ03-X2进行攻毒,收集斑马鱼的肠道和肾脏组织提取RNA,逆转录成cDNA,通过Real-time RT-PCR检测相关基因的表达。结果表明,24h后CpG-ODN2006组的免疫保护率高达70.0%;相关免疫基因表达检测结果发现CpG-ODN 2006组肠道中lysozyme、Myd88、tlrs和tnf的mRNA水平显著下调,而il1b和il10则显著上调;肾脏中的基因表达差异更为明显:lysozyme显著上调,il1b、myd88、tnf、ifng1-2、il10、il22均显著下调。结果表明CpG-ODN 2006通过调节斑马鱼肾脏及肠道中非特异性免疫相关基因的表达,维持免疫系统的平衡,增强抗创伤弧菌FJ03-X2感染的能力。Groups of zebra fish were injected with either PBS as controlor synthetic CpG-ODN 2006 for treatment,and 48 hours later,challenged by Vibrio vulnificus FJ03-X2 infection in this study.The immune protection of the treatment group reached a rate of 70.0%in 24 hours.RNA was extracted from the intestinal and kidney tissues and then reverse transcribed into cDNA.The mRNAs of lysozyme,Myd88,tlrs and tnf in the guts of the treated fish were significantly downregulated,while those of il1b and il10,significantly upregulated,as detected by Real-time RT-PCR.The differences on gene expression in kidney were even more apparent as the expression of lysozyme significantly upregulated,and those of il1b,myd88,tnf,ifng1-2,il10 and il22 significantly downregulated.It appeared that the CpG-ODN 2006 injection afforded the zebra fish to vitalize its immune system through regulating the expressions of non-specific immunity-related genes in the kidney and intestinesto ward off the viral attack.

关 键 词：斑马鱼 CPG-ODN 创伤弧菌 非特异性免疫 

分 类 号：S941[农业科学—水产养殖]