基于MnO_2纳米线-还原石墨烯复合修饰电极的多巴胺电化学检测  

Electrochemical Detection of Dopamine Based on MnO_2 Nanowires/Reduced Graphene Oxide Composites Modified Glassy Carbon Electrode

作　　者：贺全国 梁静 李广利 邓培红 刘军 刘晓鹏 HE Quan-Guo;LIANG Jing;LI Guang-Li;DENG Pei-Hong;LIU Jun;LIU Xiao-Peng(College of Life Sciences and Chemistry,Hunan University of Technology,Zhuzhou 412007 China;Department of Chemistry and Material Science,Hengyang Normal University,Hengyang 421008 China)

机构地区：[1]湖南工业大学生命科学与化学学院,株洲412007 [2]衡阳师范学院化学与材料科学学院,衡阳421008

出　　处：《分析化学》2018年第3期438-445,共8页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.61703152);湖南省自然科学基金项目(No.2016JJ4010);衡阳师范学院省级平台开放基金项目(No.GD16K02);湖南工业大学博士学科建设项目资助~~

摘　　要：通过电化学还原法制备MnO_2纳米线/还原石墨烯复合修饰电极(MnO_2-RGO/GCE),用于多巴胺(DA)的检测。采用扫描电镜和X-射线粉末衍射对不同的修饰电极微观形貌进行了表征,优化了电化学还原条件和测定DA实验条件。此外,还研究DA在裸电极及RGO或MnO_2-RGO修饰电极上的循环伏安响应。MnO_2-RGO/GCE复合修饰电极实现AA、DA和UA氧化峰的有效分离,AA-DA和DA-UA的氧化峰电位差分别为268和128 m V。检测DA的线性范围为0.06~1.0μmol/L和1.0~80μmol/L,检出限为1.0 nmol/L(S/N=3)。制备的MnO_2-RGO/GCE成功用于人血清样品的多巴胺含量分析。MnO2 nanowires-reduced graphene oxide composite(MnO2-RGO)was used to modify glassy carbon electrodes(GCE)and applied for the electrochemical determination of dopamine(DA).The microstructure of MnO2 nanowires and MnO2-RGO nanocomposite material was characterized by scanning microscope and X-ray powder diffraction.Then the electrochemical reduction condition for preparing MnO2-RGO/GCE and experimental conditions for determining DA were optimized systematically.The electrochemical behavior of DA on the bare electrode and RGO or MnO2-RGO modified electrodes was also investigated in pH 3.5 phosphate buffer solution(PBS)by cyclic voltammetry.The results shows that the oxidation peaks of ascorbic acid(AA),dopamine(DA)and uric acid(UA)can be well separated and the peak to peak separations were 268 mV(AA-DA)and 128 mV(DA-UA),respectively.Moreover,the linear response ranges for the determination of DA were 0.06-1.0μmol/L and 1.0-80μmol/L with the detection limit of 1.0 nmol/L(S/N=3).The proposed method has been applied to the determination of dopamine in human blood serum sample with satisfactory results.

关 键 词：MnO2纳米线 还原石墨烯 修饰电极 多巴胺 电催化氧化 

分 类 号：O657.1[理学—分析化学] R446.1[理学—化学]