烤烟主要农艺性状变异特征以及与烟碱含量的相关分析  

Variation of Main Agronomic Traits and Correlation Analysis of Nicotine Content in Flue-cured Tobacco

作　　者：尹鹏嘉[1,2] 任民[1] 孙鑫 周世奇 杨爱国[1] 罗成刚[1] 陈爱国[1] 文柳璎[1] YIN Pengjia;REN Min;SUN Xin;ZHOU Shiqi;YANG Aiguo;LUO Chenggang;CHEN Aiguo;WEN Liuying(Tobacco Research Institute,Key Laboratory of Tobacco Genetic Improvement and Biotechnology,Chinese Academy of Agricultural Sciences,Qingdao 266101,China;Graduate School,CAAS,Beijing 100081,China;The Office for the Protection of New Varieties of Plants,MOA,P.R.,Beijing 100081,China)

机构地区：[1]中国农业科学院烟草研究所,中国农业科学院烟草遗传改良与生物技术重点开放实验室,青岛266101 [2]中国农业科学院研究生院,北京100081 [3]农业部植物新品种保护办公室,北京100081

出　　处：《中国烟草科学》2018年第1期10-16,共7页Chinese Tobacco Science

基　　金：中国农业科学院科技创新工程(ASTIP-TRIC01)；山东省自然科学基金(ZR2016CB39)；中国农业科学院烟草研究所青年科学基金(2015A01)；国家烟草专卖局烟草基因组计划重大专项(110201301008)

摘　　要：为加快高烟碱烤烟品种的田间选育,以116份烤烟种质资源为供试材料,连续种植4年,于烟叶成熟期测定其13个农艺性状,采用因子分析、逐步回归分析、二次响应面分析等方法探讨了烤烟烟碱含量与农艺性状之间的相关性。结果表明,烤烟主要农艺性状变异丰富,变异系数达5.64%~16.96%。烟碱含量变异系数达到24.3%。简单相关分析表明,烟碱含量与节距呈极显著正相关,与茎围、叶数呈显著负相关。通过因子分析,明确了与烟碱含量相关的主要农艺性状包括烟叶长宽比因子、叶数因子、叶长因子、株高因子。采用逐步回归分析法,建立了烤烟主要农艺性状与烟碱含量之间的回归模型,经统计学检验,达到极显著水平。通过二次响应面分析,展现了腰叶长、下二棚叶长与烟碱含量之间的动态变化关系,当下二棚叶长63.7 cm,腰叶长58.7 cm,烟株的烟碱含量稳定且有极小值。烟叶长宽比较小、节距较大、叶数较少和株高较高的烤烟,烟碱含量较高,因此,在选育不同烟碱含量的烤烟品种时,应综合考虑。In order to increase selection efficiency of tobacco breeding,we carried out an analysis of relationship between nicotine content and agronomic traits.In this study,13 agronomic traits of 116 tobacco varieties were measured in 4 consecutive years.Correlation analysis,factor analysis,stepwise selection analysis and quadratic response surface analysis were used to explore the relationship between nicotine content and these traits.The results showed that variations in main agronomic traits of tobacco varieties were abundant with the coefficients of variation ranging from 5.64%-16.96%and the variation coefficient of nicotine content was 24.3%.Simple correlation analysis showed that nicotine content was significantly positively correlated with the internodal distance,and negatively correlated with stem girth and leaf number.Factor analysis showed that leaf length-width ration factors,leaf number factors,leaf length factors,plant height factors were largely responsible for nicotine content.Regression models,which successfully forecasted the relationships between agronomic traits and nicotine content were established by Stepwise selection method analysis.Statistical test was performed and the results showed that the correlation coefficient was significant.Furtherly,second response surface analysis revealed the dynamical relationship of nicotine content between a leaf length and middle leaf length.When bottom leaf length is 63.7 cm and middle leaf length is 58.7 cm,the nicotine content of a tobacco plant is stable and has a minimal value.Nicotine content will increase when tobacco plants have a smaller leaf length-width ratio,bigger internodal distance,less leaf number,and higher plant height.Therefore,when breeding for flue-cured tobacco varieties with different nicotine contents,breeders can use these agronomic traits as reference.

关 键 词：烤烟 烟碱含量 相关分析 农艺性状 

分 类 号：S572.03[农业科学—烟草工业]