基于cpDNA和nrITS对濒危植物掌叶木的基因检测  

Identification of endangered plant of Handeliodendron bodinieri by cpDNA and nrITS sequence analyses

作　　者：滕婕华 倪敏 李超群 李鹏伟 谢国文[1] TENG Jiehua;NI Min;LI Chaoqun;LI Pengwei;XIE Guowen(College of Life Sciences,Guangzhou University,Guangzhou 510006,China;Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China)

机构地区：[1]广州大学生命科学学院,广东广州510006 [2]中国科学院植物研究所,北京100093

出　　处：《北方农业学报》2017年第3期23-28,共6页Journal of Northern Agriculture

基　　金：广州大学大学生创新训练项目(CX2016010);全国大学生"挑战杯"课外科技活动项目

摘　　要：通过使用叶绿体基因和核糖体基因,对掌叶木进行基因检测研究。采用基因测序方法,用叶绿体基因(cpDNA)包括rpl32-trnL,trnH-psb A,trnL-F,trnS-G,trnT-L,mat K,trnH-trnK及rps16和核糖体核酸内转录间隔区基因(nrDNA ITS)包括ITS1-4及ITS5f-2r的序列,进行基因测序。通过序列比对,发现96个掌叶木个体10对引物扩增的基因片段序列几乎完全一致。遗传距离变化范围为0.000~0.001。选用七叶树作为外类群,96个掌叶木个体基本处于同一分支上,几乎无信息位点。因此,通过使用cpDNA和nrITS基因检测的方法未能划分掌叶木分类群,该基因检测法不合适应用于掌叶木分类。Ten DNA regions were sequenced for gene sequencing study on Handeliodendron bodinieri.These ten DNA regions were chloroplast DNA included rpl32-trnL,trnH-psbA,trnL-F,trnS-G,trnT-L,matK,trnH-trnK and rps16,nrDNA ITS(nuclear ribosomal DNA internal transcribed spacer)included ITS1-4 and ITS5f-2r.By sequence alignment,it showed 10 fragments of 96 individual gene sequences were almost identical.Genetic distance ranged from 0.000~0.001.Horse Aesculus chinensis Bunge was selected as outside groups,96 individuals on the same branch,little bits of information.So,the genetic sequencing method was difficult to apply by using the method of cpDNA and nrITS gene sequencing to divide the Handeliodendron bodinieri clusters.

关 键 词：掌叶木 濒危植物 CPDNA NRITS 基因测序法 

分 类 号：Q948[生物学—植物学] S792[农业科学—林木遗传育种]