乙型病毒性肝炎患者血清HBV DNA与HBeAg、HBsAg的相关性分析  

Analysis of correlation between serum HBV DNA,HBeAg and HBsAg in patients infected with Hepatitis B virus

作　　者：侯玲[1] 刘韧[1] 龙训琴 邓红艳[2] 管雯[2] HOU Ling;LIU Ren;LONG Xunqin;DENG Hongyan;GUAN Wen(Department of Clinical Laboratory,Chengdu Western Hospital, Chengdu,Sichuan 610071,China;Department of Clinical Laboratory,Affiliated Hospital of Chengdu University,Chengdu,Sichuan 610000,China)

机构地区：[1]四川省成都市西区医院检验科,610071 [2]成都大学附属医院检验科,成都610000

出　　处：《检验医学与临床》2018年第7期936-939,共4页Laboratory Medicine and Clinic

摘　　要：目的分析乙型病毒性肝炎患者血清乙型肝炎病毒核酸(HBV DNA)与乙型肝炎病毒e抗原(HBeAg)、乙型肝炎病毒表面抗原(HBsAg)的相关性。方法选取四川省成都市西区医院98例乙型病毒性肝炎患者作为研究对象,以不同水平的HBV DNA对HBeAg、HBsAg进行分组,通过荧光定量聚合酶链反应技术对其血清HBV DNA进行检测,并通过化学发光微粒子免疫法对其血清学标志物进行检测,分析HBV DNA与HBeAg、HBsAg的相关性。结果HBV DNA与HBeAg呈显著正相关(r=0.46,P<0.05),各组间HBeAg水平比较,差异有统计学意义(P<0.05);HBV DNA与HBsAg呈显著正相关(r=0.35,P<0.05),各组间HBsAg水平比较,差异无统计学意义(P>0.05)。丙氨酸氨基转移酶(ALT)水平高于40U/L时,各组间HBV DNA水平比较差异有统计学意义(P<0.05)。结论高水平的HBeAg与HBV DNA存在密切关系,而因HBsAg检测线性范围较小,尚未能够说明其与HBV DNA的相关性,同时血清HBV DNA水平较高时,乙型病毒性肝炎患者ALT水平较高,提示患者肝损伤的可能较为严重。Objective To analyze the correlation between serum hepatitis B virus nucleic acid(HBV DNA)and hepatitis B virus e antigen(HBeAg),hepatitis B virus surface antigen(HBsAg)in patients infected with hepatitis B virus.Methods A total of 98 patients infected with hepatitis B virus were selected in Chengdu Western Hospital.The serum HBV DNA was detected by fluorescence quantitative PCR technique,the serological markers were detected by CMIA method,and the correlation between HBV DNA and HBeAg,HBsAg were analyzed.Results There were significant differences found in HBeAg among different HBV DNA levels(P<0.05),and the correlation between HBV DNA and HBeAg was statistically significant(r=0.46,P<0.05).No significant difference of HBsAg found among different HBV DNA levels(P>0.05),while the relationship between HBV DNA and HBsAg was statistically significant(r=0.35,P<0.05).There were significant differences of HBV DNA levels among the groups when glutamate transaminase(ALT)was higher than 40 U/L.Conclusion There is a close relationship between high concentration of HBeAg and HBV DNA.But it can′t explain the association between HBsAg and HBV DNA on account of the small linear range of HBsAg detection.Furthermore,when the serum HBV DNA level is high,and the ALT level is high in patients infected with hepatitis B virus,which suggests that the liver injury may be serious.

关 键 词：乙型病毒性肝炎 乙型肝炎病毒核酸 乙型肝炎病毒E抗原 乙型肝炎病毒表面抗原 相关性 

分 类 号：R512.62[医药卫生—内科学]