白细胞介素-6快速定量荧光免疫层析试纸条的制备及特性研究  

Significance of rapid and quantitative detection of Interleukin-6 based on fluorescence immunochromatography assay

作　　者：张文琪 何小维[1] 李凯 刘晓云 李文美 ZHANG Wenqi;HE Xiaowei;LI Kai;LIU Xiaoyun;LI Wenmei(College of Food Science and Technology,South China University of Technology, Guangzhou,Guangdong 510000,China;Guangzhou Wondfo Biotech Co.Ltd., Guangzhou,Guangdong 510000,China;School of Public Health and Preventive Medicine,Southern Medical University,Guangzhou,Guangdong 510000,China)

机构地区：[1]华南理工大学食品科学与技术学院,广州510000 [2]广州万孚生物技术股份有限公司,广州510000 [3]南方医科大学公共卫生与预防医学学院,广州510000

出　　处：《检验医学与临床》2018年第7期958-961,共4页Laboratory Medicine and Clinic

摘　　要：目的利用免疫荧光技术研制一种快速、高灵敏度定量检测血清中白细胞介素-6(IL-6)的方法。方法本研究对试剂盒的灵敏度、准确度、特异性和稳定性进行了初步的性能评价。结果本方法的标准曲线线性关系良好,r^2=0.965,检测线和定量限分别为8.49、25.28pg/mL,添加回收试验测得其回收率平均值分别为96.51%、102.46%、103.06%,且制备试纸条的批间和批内精密度均小于15.00%,与7种竞争物交叉反应率小于0.10%。结论本试验制备的IL-6定量荧光免疫层析试纸条的灵敏度高,特异性好,稳定性好,有较为广泛的应用前景。Objective To study a rapid and quantitative method to detect Interleukin-6(IL-6)by immunofluorescence technique.Methods The sensitivity and accuracy of strip associated with precision,specificity,stability properties were preliminarily evaluated.Results The standard curve showed a good linear(r 2=0.965),LOD and LOQ of the strip were 8.49 pg/mL and 25.28 pg/mL.The average recoveries were 96.51%,102.46%and 103.06%,respectively.The precision of intra-assay and inter-assay were less than 15.00%.And the cross-reaction with 7 competitors was less than 0.10%.Conclusion IL-6 immunochromatographic strip offered by our lab has better sensitivity,specificity and stability,as well as wide application prospect.

关 键 词：白细胞介素-6 免疫荧光定量层析法 检测 

分 类 号：R318.6[医药卫生—生物医学工程]