HPLC法同时测定桑白皮中6种活性成分的含量  

Content Determination of 6 Active Components in Morus alba by HPLC

作　　者：陈志永 蒙麦侠[1] 杨媛媛 杜霞[1] 杨智峰[1] 任慧[1] CHEN Zhiyong;MENG Maixia;YANG Yuanyuan;DU Xia;YANG Zhifeng;REN Hui(Shaanxi Academy of TCM,Xi’an 710003,China;Xi’an Institute for Food and Drug Control,Xi’an 710054,China)

机构地区：[1]陕西省中医药研究院,西安710003 [2]西安市食品药品检验所,西安710054

出　　处：《中国药房》2018年第7期911-914,共4页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81603264)

摘　　要：目的:建立同时测定桑白皮中新绿原酸、桑皮苷A、绿原酸、紫云英苷、桑根酮C和桑辛素等6种活性成分含量的方法,为完善桑白皮的质量控制标准提供参考。方法:采用高效液相色谱法。色谱柱为Agilent 5 TC-C_(18),流动相为乙腈-0.1%甲酸(梯度洗脱),流速为1 mL/min,检测波长为280 nm。结果:新绿原酸、桑皮苷A、绿原酸、紫云英苷、桑根酮C和桑辛素检测质量浓度的线性范围分别为0.001 06~0.042 4、0.001 67~0.066 8、0.007 95~0.318、0.001 65~0.066 0、0.005 00~0.200和0.001 24~0.049 6 mg/mL(r均≥0.999 6),定量限分别为0.11、0.14、0.81、0.17、0.45和0.12μg/m L,检测限分别为0.04、0.05、0.41、0.07、0.18和0.04μg/m L,精密度试验的RSD分别为0.26%、0.31%、0.24%、0.27%、0.36%和0.44%(n=6),稳定性试验的RSD分别为0.68%、0.54%、0.62%、0.53%、0.41%和0.73%(n=6),平均方法回收率为99.1%、98.8%、98.8%、98.4%、98.5%和99.9%(RSD为0.5%~1.5%,n=9)。结论:该法简便、准确,可用于桑白皮中6种活性成分的同时测定。OBJECTIVE:To establish the method for the simultaneous determination of content of 6 active components as neochlorogenic acid,mulberroside A,chlorogenic acid,astragalin,sanggenon C and morusin in Morus alba,and to provide reference for improving quality control standard of M.alba.METHODS:HPLC method was adopted.The determination was performed on Agilent 5 TC-C18 with mobile phase consisted of acetonitrile-0.1%formic acid(gradient elution)at the flow rate of 1 mL/min.The detection wavelength of 280 nm.RESULTS:The mass concentration linear range of neochlorogenic acid,mulberroside A,chlorogenic acid,astragalin,sanggenon C and morusin were 0.001 06-0.042 4,0.001 67-0.066 8,0.007 95-0.318,0.001 65-0.066 0,0.005 00-0.200 and 0.001 24-0.049 6 mg/mL,respectively(all r≥0.999 6);the limits of quantitation were 0.11,0.14,0.81,0.17,0.45 and 0.12μg/mL,respectively;the limits of detection were 0.04,0.05,0.41,0.07,0.18 and 0.04μg/mL,respectively;RSDs of precision test were 0.26%,0.31%,0.24%,0.27%,0.36%and 0.44%(n=6),respectively;RSDs of stability test were 0.68%,0.54%,0.62%,0.53%,0.41%and 0.73%(n=6),respectively;average method recovery rates were 99.1%,98.8%,98.8%,98.4%,98.5%and 99.9%(RSDs were 0.5%-1.5%,n=9),respectively.CONCLUSIONS:The method is simple,accurate,and can be used for simultaneous determination of 6 active components in M.alba.

关 键 词：桑白皮 高效液相色谱法 新绿原酸 桑皮苷A 绿原酸 紫云英苷 桑根酮C 桑辛素 含量测定 

分 类 号：R284[医药卫生—中药学]