大豆种子老化的转录组学分析  

Transcriptome analysis of aging soybean seeds

作　　者：秦雯婷 杨才琼[1,2] 邓俊才 Nasir Iqbal 杨峰 王小春 杨文钰 刘江 QIN Wenting;YANG Caiqiong;DENG Juncai;YANG Feng;WANG Xiaochun;YANG Wenyu;LIU Jiang(Key Laboratory of Crop Ecophysiology and Farming System in Southwest,Ministry of Agriculture,Chengdu 611130,China;Institute of Ecological Agriculture,Sichuan Agricultural University,Chengdu 611130,China;Sichuan Engineering Research Center for Crop Strip Intercropping,Chengdu 611130,China)

机构地区：[1]农业部西南作物生理生态与耕作重点实验室,成都611130 [2]四川农业大学生态农业研究所,成都611130 [3]四川省作物带状复合种植工程技术研究中心,成都611130

出　　处：《东北农业大学学报》2018年第3期1-9,共9页Journal of Northeast Agricultural University

基　　金：国家重点研发计划专项(2016YFD0300209)；国家自然科学基金项目(31401329，31301277)；中国博士后科学基金项目(2014M560724，2017T100707)

摘　　要：大豆种子储存过程易老化劣变,研究大豆种子老化分子机制有助于耐储藏大豆品种遗传改良。文章对不同耐储性大豆品种老化种子作转录组测序分析,结果表明,储存2~4年后,易老化大豆"JP16"和耐储型大豆"JP6"分别有1 683个和832个差异转录基因,皆以上调为主。基因功能注释显示,这些基因主要富集于碳水化合物合成、水分损失、线粒体机能改变及蛋白质降解等生物过程。另外,"JP16"响应蛋白质泛素化和脂肪酸氧化基因上调,花青素、木质素合成相关基因下调;而"JP6"响应原花青素、异黄酮合成及活性氧水平调控相关基因上调,蜡质代谢相关基因下调。大豆种子通常随储存时间延长,内部碳水化合物降解,蛋白变性,扰乱胞内水分正常代谢,加剧活性氧积累,种子老化加剧。耐储型大豆种子可通过积累蜡质、原花青素、异黄酮等保护性成分,增强对活性氧调控提高细胞壁强度和抗氧化能力,延缓老化。研究结果为利用分子手段提高大豆种子耐储性能提供参考。Soybean seeds are vulnerable to aging and deterioration during storage.Deep investigation of molecular mechanisms of soybean seed aging will be helpful for the genetic improvement in the storage stability of soybean cultivars.In this study,two soybean cultivars with different storage-tolerance,"JP6"and"JP16",were used as the experimental materials to investigate the transcriptome changes in response to storage by RNA-Seq technology.The results showed that there were 1683 differentially expressed genes in"JP16"and 832 differentially expressed genes in"JP6"in the aging process of soybean seed,most of which were up regulated.Gene ontology analyses showed that differentially expressed genes were enriched in carbohydrate biosynthetic process,response to water deprivation,mitochondrial electron transport,protein phosphorylation and so on.Moreover,differentially expressed genes in response to protein monoubiquitination and fatty acid omega-oxidation were up-regulated,but genes in response to regulation of anthocyanin and lignin metabolic process were down-regulated in"JP16".Similarly,genes in response to proanthocyanidin and isoflavonoid biosynthetic process,and decreased oxygen levels were up regulated,but genes in response to wax metabolic process were down regulated.The accumulation of carbohydrate decreased in soybean seeds during storage,meanwhile,the albuminous degeneration and intracellular water balance disturbed,and the accumulation of reactive oxygen species increased.The above factors made an aggravated seed aging level.During storage,there were significant differences exist in genes between"JP6"and"JP16",of which"JP6"had more wax,proanthocyanidin,isoflavonoid and other protective components to increase antioxidant and cell wall strength via regulation of reactive oxygen species level,resulting in low sensitivity to aging.

关 键 词：大豆 储存 老化 转录组 

分 类 号：S330.2[农业科学—作物遗传育种]