外施Ca^(2+)对盐胁迫下珠美海棠组培苗光合特性的影响  

Effects of Exogenous Ca^(2+) on Photosynthetic Characteristics of Tissue Cultured Seedlings of Malus zumi under Salt Stress

作　　者：秦家慧 李颖华 范航伟 孙敏 彭立新 QIN Jiahui;LI Yinghua;FAN Hangwei;SUN Min;PENG Lixin(College of Horticulture and Landscape,Tianjin Agricultural University,Tianjin 300384,China)

机构地区：[1]天津农学院园艺园林学院,天津300384

出　　处：《天津农业科学》2018年第4期1-5,共5页Tianjin Agricultural Sciences

基　　金：国家自然科学基金项目(30671440)

摘　　要：为研究Ca^(2+)对盐胁迫下植株伤害的缓解作用,以珠美海棠组培苗为材料,采用盆栽法研究外施Ca^(2+)对盐胁迫下珠美海棠光合特性的影响。采用双因素随机区组设计,NaCl设置0,100,200,300 mmol·L^(-1)4个浓度梯度,Ca^(2+)设置0,5,10,15,20 mmol·L^(-1)5个浓度梯度,对各处理珠美海棠色素和光合指标进行测定和分析。结果表明:(1)单一NaCl胁迫和外施Ca^(2+)处理均可使珠美海棠叶绿素a和b含量呈先升高后下降的趋势,其中在Na Cl和Ca^(2+)浓度分别达到200 mmol·L^(-1)和15 mmol·L^(-1)时达到最高值;(2)单一Na Cl胁迫可使珠美海棠Pn、Gs和Tr呈先升高后下降的趋势,Ci趋势与三者相反,其中在Na Cl浓度达到100 mmol·L^(-1)时Pn、Gs和Tr达到最高值而Ci达到最低值;(3)在Na Cl高浓度即200 mmol·L^(-1)和300 mmol·L^(-1)处理,外施适宜浓度Ca^(2+)对盐胁迫下珠美海棠光合指标有缓解作用,并分别以外施15,10 mmol·L^(-1) Ca^(2+)效果最佳。研究可为珠美海棠在滨海盐碱地的引种栽培提供参考和借鉴。To study the mitigation effect of Ca2+on plant injury under salt stress,the effects of additional Ca2+on photosynthetic characteristics of Malus zumi under salt stress were studied by pot culture method.Two factors randomized block design was used,which included 4 NaCl concentration gradient(0,100,200,300 mmol·L-1),and 5 Ca2+concentration gradient(0,5,10,15,20 mmol·L-1),the pigment and photosynthetic indices of M.zumi were measured and analyzed.The results showed as follows:(1)under single NaCl stress or Ca2+treatments,the chlorophyll a and b contents of Malus zumi were increased and then decreased and the maximum was at 200 mmol·L-1 and 15 mmol·L-1,respectively;(2)Under single NaCl stress,the Pn,Gs and Tr of Malus zumi were also increased and then decreased while the trend of Ci was contrary,the maximum of Pn,Gs and Tr or the minimum of Ci was at NaCl 100 mmol·L-1;(3)When NaCl concentration was 200 mmol·L-1 and 300 mmol·L-1,the suitable Ca2+could relieve the injury of salt on photosynthetic indices of Malus zumi,and the optimum Ca2+were 15 and 10 mmol·L-1 respectively.The results could provide reference for introduction and cultivation of Malus zumi in coastal saline-alkali land.

关 键 词：珠美海棠 盐胁迫 外施Ca2+ 光合特性 

分 类 号：S661.4[农业科学—果树学]