新型复合磷酸钙镁基水门汀的生物相容性  

Biocompatibility of a novel calcium-magnesium phosphate composite cement in dentistry

作　　者：沈晴昳[1] 李国强[1] 李恺[2] Shen Qing-yi;Li Guo-qiang;Li Kai(Department of Prosthodontics,Shanghai Stomatological Disease Center,Shanghai Stomatology Hospital of Fudan University,Shanghai 200031,China;Key Laboratory of Inorganic Coating Materials,Shanghai Institute of Ceramics,Chinese Academy of Sciences,Shanghai 200025,China)

机构地区：[1]上海市口腔病防治院,复旦大学附属上海市口腔医院修复科,上海市200031 [2]中国科学院上海硅酸盐研究所无机涂层重点实验室,上海市200050

出　　处：《中国组织工程研究》2018年第2期241-247,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81300917)

摘　　要：背景:课题组将磷酸钙、磷酸镁、硅酸钙、碳酸铋多种材料复合,研制出一种新型的无机复合材料用作修复髓室底穿孔,前期研究显示该复合材料具有良好的理化机械性能。目的:分析磷酸钙-磷酸镁-硅酸钙-碳酸铋材料的生物相容性。方法:(1)细胞毒性实验:通过CCK-8比色法测定磷酸钙-磷酸镁-硅酸钙-碳酸铋材料对L929细胞增殖的影响,评价材料的细胞毒性;(2)溶血实验:在兔抗凝血中分别加入受试样品浸提液、生理盐水与蒸馏水,检测溶血率;(3)急性全身毒性实验:将受试样品浸提液、生理盐水分别经小鼠尾静脉注入实验组、对照组动物,观察24,48,72 h小鼠一般情况,并记录各个时间段的体质量;(4)Ames实验:采用标准平板掺入法,计数组氨酸营养缺陷型鼠伤寒沙门氏菌TA97,TA98,TA100,TA102标准测试菌株在受试样品浸提液下培养72 h的回变菌落数。加S-9混合液作为体外代谢活化系统。结果与结论:新型无机复合磷酸钙-磷酸镁-硅酸钙-碳酸铋材料的体外细胞毒性为0级,急性溶血、急性全身毒性和Ames实验结果均为阴性,该材料具有良好生物相容性与安全性。BACKGROUND:In our preliminary experiments,calcium phosphate cement,magnesium phosphate cement,calcium silicate and bismuth were mixed to manufacture a novel inorganic composite material,which would be used to repair furcal perforation.This novel composite cement has been confirmed to have excellent physiochemical and mechanical properties.OBJECTIVE:To study the biocompatibility of calcium phosphate-magnesium phosphate-calcium silicate-bismuth cement(CMSBC).METHODS:Cytotoxicity test:Relative growth rate of L929 cells cultured in CMSBC extract was analyzed using cell counting kit-8 assay.Acute hemolysis test:The leaching solution of test samples,saline and distilled water were added into the rabbit anti-clotting,to detect the rate of hemolysis.Acute systemic toxicity test:CMSBC lead liquor and normal saline were respectively injected via the tail vein into the mice in experimental group and control group,respectively.The general situation of mice was observed at 24,48,72 hours respectively and body weight change was valued.Ames test:In the flat-plate incorporation test of Salmonella typhimurium,the average number of spontaneous revertants of TA97,TA98,TA100 and TA102 of CMSBC leaching liquor was calculated after incubation at 37℃for 72 hours.S-9 mixture was added to the plates as an in vitro activation system.RESULTS AND CONCLUSION:The cytotoxicity of CMSBC in vitro was grade 0.Negative results from the acute hemolytic test,acute systemic toxicity test and Ames test of CMSBC indicate that this novel inorganic composite material has good biocompatibility and biosecurity.

关 键 词：磷酸钙类 生物相容性材料 材料试验 组织工程 

分 类 号：R318[医药卫生—生物医学工程]