成年似鲇高原鳅外周血细胞及血细胞发生的观察  

Observersional researches on peripheral blood cells and morphogenesis of haematopoietic cells in Triplophysa siluroides

作　　者：赵柳兰[1] 代敏[1] 杨淞[1] 吴应斌 汤巨平 杨世勇[1] 杜宗君[1] ZHAO Liulan;DAI Min;YANG Song;WU Yingbin;TANG Juping;YANG Shiyong;DU Zongjun(College of Animal Science and Technology,Sichuan Agricultural University,Chengdu 611130,China;Breed Farm of Mountain Loach,E’meishan 614224,Sichuan,China)

机构地区：[1]四川农业大学动物科技学院,成都611130 [2]四川省似鲇高原鳅省级原种场,四川峨眉山614224

出　　处：《淡水渔业》2018年第2期16-23,31,共9页Freshwater Fisheries

基　　金：四川省十三五水产育种攻关项目之高品质鱼类选育;育种材料与方法创新(16ZC2847);四川农业大学双支计划(03571778;03572417)

摘　　要：通过对成年似鲇高原鳅(Triplophysa siluroides)外周血细胞涂片及中肾、肝脏和脾脏组织的印片进行瑞氏(Wright’s)、过碘酸雪夫氏(PAS)和苏丹黑B(SBB)染色,观察各类血细胞形态、大小、分布及细胞化学特征。结果表明:似鲇高原鳅血细胞可分为红细胞、粒细胞、单核细胞、淋巴细胞和血栓细胞,各类血细胞的发生(除血栓细胞)均可分为原始、幼稚和成熟三个阶段。外周血中红细胞比例最多,为97.56%,白细胞中血栓细胞数量最多占52.86%,居白细胞的首位;三种粒细胞中嗜中性粒细胞数量最多占30.25%。粒细胞的主要发生场所在中肾占45.18%,红细胞的主要发生场所在脾脏,占71.62%,肝脏中未见原始血细胞。在中肾中同时可见嗜酸性、嗜碱性和嗜中性三种粒细胞的发生。各阶段的白细胞PAS染色均显示阳性,但只有各阶段的粒细胞SBB染色显示阳性。The methods of Wright’s,Periodic acid-Schiff(PAS)and Sudan back B(SBB)staining were used to dye the peripheral blood cells and the kidney,spleen and liver in Triplophysa siluroides to observe the morphology,size,distribution and cytochemical characteristics of various blood cells.The results showed that the blood cells could bedevided into 5 types as erythrocytes,granulocytes,monocytes,lymphocytes and thrombocytes.The process of morphogenesis in all kinds of blood cells except thrombocytes included primitive,native and mature-three stages.The largest proportion of peripheral blood was red blood cells,which shared over 97.56%.Thrombocytes had a high percentage of 52.8%in white blood cells.Neutrophilic granulocytes were the predominant cells among the three granulocytes,accounting for 30.25%.The main haemopoietic tissue of granulocytes and red blood cells were the body kidney and spleen,accounting for 45.18%and 71.62%,respectively.The primitive blood cells were not found in the liver.The development of eosinophils,basophils and neutrophils could be observed simultaneously in the kidney.PAS of white blood cells in each stage showed positive staining,and only granulocytes in every developmental stages showed SBB staining positive.

关 键 词：似鲇高原鳅(Triplophysa siluroides) 外周血细胞 血细胞的发生 造血组织 细胞化学 

分 类 号：S917.4[农业科学—水产科学]