基于SLAF-seq技术的油茶SNP位点开发及杂交后代早期鉴定  

Development of SNP Markers of Camellia oleifera Based on SLAF-seq and Early Identification of Hybrids

作　　者：刘凯[1] 王东雪[1] 江泽鹏 莫健芳 曾雯珺[1] 张乃燕[1] 梁国校[1] LIU Kai;WANG Dong-xue;JIANG Ze-peng;MO Jian-fang;ZENG Wen-jun;ZHANG Nai-yan;LIANG Guo-xiao(Guangxi Forestry Research Institute,Improved Variety and Cultivation Engineering Research Center of Oil-tea Camellia in Guangxi,Guangxi Key Laboratory of Special Non-wood Forest Cultivation&Utilization,Nanning 530002,China;State Beijianghe Forest Farm of Miao Autonomous County of Rongshui,Rongshui,Guangxi 545300,China)

机构地区：[1]广西壮族自治区林业科学研究院广西油茶良种与栽培工程技术研究中心广西特色经济林培育与利用重点实验室,南宁530002 [2]融水苗族自治县国有贝江河林场,广西融水545300

出　　处：《广西林业科学》2018年第1期13-17,共5页Guangxi Forestry Science

基　　金：广西自然科学基金项目(2015GXNSFAA139075;2017JJ130004);广西特色经济林培育与利用重点实验室项目(16-A-01-01)

摘　　要：为了开发大量的分子标记用于油茶(Camellia oleifera)亲本杂交后代的早期鉴定,本研究采用SLAF-seq(Specific-Locus Amplified Fragment Sequencing)技术对9个油茶样品(包括5个亲本及其4个子代单株)进行测序并开发SNP标记。最终根据多态性SLAF标签,开发得到1 644 616个群体SNP。基于群体SNP的亲子关系分析结果表明,岑软(Cenxi soft-branch C.oleifera)ZJ11为岑软2号与岑软11号的杂交后代,岑软ZJ14为岑软11号与岑软22号的杂交后代,岑软ZJ24为岑软11号与岑软24号的杂交后代,优31则是岑软11号与岑软23号的杂交后代。In order to develop a large number of molecular markers for early identification of Camellia oleifera hybrids,9 samples of Camellia oleifera(including 5 parents and 4 progenies)were sequenced by SLAF-seq technology and SNP markers were developed.Finally,a total of 1 644 616 population SNPs were developed based on the polymorphic SLAFs.Paternity analysis of the 9 samples ofC.oleifera based on the population SNPs revealed that ZJ11,ZJ14 and ZJ24 was hybrid progenies of Cenruan 2 and Cenruan 11,CR-11 and CR-22,CR-11 and CR-24,respectively,while You 31 was a hybrid progeny of CR-11 and CR-23.

关 键 词：油茶 SLAF-seq 分子标记 SNP 

分 类 号：S794.4[农业科学—林木遗传育种]