不同生物素水平对3T3-L1脂肪细胞脂肪合成相关基因转录表达的影响  

The Impact of Various Biotin Levels on Adipogenetic Relevant Gene Transcription and Expression of 3T3-L1 Adipocytes

作　　者：雒诚龙 邵伟[1,2] 任万平 赵艳坤[1] 王东海[1] 余雄[2] LUO Cheng-long;SHAO Wei;REN Wan-ping;ZHAO Yan-kun;WANG Dong-hai;YU Xiong(Collage of Animal Science,Xinjiang Agricultural University,Urumqi 830052,China;Xinjiang Key Laboratory of Meat and Milk Production Herbivore Nutrition,Urumqi 830052,China)

机构地区：[1]新疆农业大学动物科学学院,乌鲁木齐830052 [2]新疆肉乳用草食动物营养重点实验室,乌鲁木齐830052

出　　处：《新疆农业科学》2018年第2期371-378,共8页Xinjiang Agricultural Sciences

基　　金：现代农业产业技术体系建设专项资金资助(CARS-37);新疆维吾尔自治区重点实验室开放课题(2015KL018);中国博士后基金委资助项目;国家自然基金项目(31560645)~~

摘　　要：【目的】诱导3T3-L1细胞分化为3T3-L1脂肪细胞,研究不同生物素水平对3T3-L1脂肪细胞脂肪合成相关基因转录表达的影响。【方法】利用三联诱导法将3T3-L1细胞经诱导分化为3T3-L1脂肪细胞。当3T3-L1脂肪细胞密集后分别采用0(对照组)、0.2、0.5、1μmol/L生物素处理,分别在12 h、24 h与48 h时检测细胞上清液中PK mRNA、GLUT-4 mRNA、FAS mRNA及ACC1 mRNA相对表达量。【结果】在试验12h时:各试验组GLUT-4、PK、ACC1 mRNA相对表达量均极显著(P<0.01)高于对照组,1μmol/L组与0.5μmol/L组FAS mRNA相对表达量极显著(P<0.01)高于对照组与0.2μmol/L组;在试验24 h时:1μmol/L组GLUT-4 mRNA与PK mRNA相对表达量显著(P<0.05)高于对照组,1μmol/L组ACC1 mRNA相对表达量极显著(P<0.01)高于对照组,0.2μmol/L组与0.5μmol/L组ACC1 mRNA相对表达量显著(P<0.05)高于对照组;在试验48 h时:1μmol/L组GLUT-4 mRNA相对表达量显著(P<0.05)高于对照组,1μmol/L组PK mRNA相对表达量极显著(P<0.01)高于对照组,0.5μmol/L组FAS mRNA相对表达量极显著(P<0.05)高于1μmol/L组,1μmol/L组FAS mRNA相对表达量显著(P<0.05)高于对照组,1μmol/L组ACC1mRNA相对表达量极显著(P<0.01)高于其它组。【结论】生物素的添加可以提升脂肪细胞GLUT-4、PK、FAS与ACC1 mRNA相对表达量,且当以1μmol/L浓度作用时对GLUT-4、PK与ACC1提升效果最佳,以0.5μmol/L浓度作用时对FAS提升效果最佳。【Objective】In this study,the purpose is to induce 3T3-L1 cells to differentiate into 3T3-L1 adipocytes and to study the effects of various biotin levels on the transcription and expression of adipose synthase related genes in 3T3-L1 adipocytes.【Method】3T3-L1 cells were induced to differentiate into 3T3-L1 adipocytes by triplex induction.When 3T3-L1 adipocytes were dense,they were treated by 0μmol/L biotin(control group),0.2,0.5 and 1μmol/L The relative expression of competitive PKmRNA,GLUT-4 mRNA,FAS mRNA and ACC1 mRNA in cell supernatant was detected at 12h,24h and 48h,respectively.【Result】Results showed that when the test time was 12 h:GLUT-4 mRNA,PK mRNA and ACC1 mRNA relative expression quantity of experimental groups was significantly(P<0.01)higher than that of control group,FAS mRNA relative expression quantities of 0.5μmol/L group and 1μmol/L group were significantly(P<0.01)higher than that of control group.When the test time was 24 h:GLUT-4 mRNA and PK mRNA relative expression quantity of 1μmol/L group was significantly(P<0.05)higher than that of control group,ACC1 mRNA relative expression quantity of 1μmol/L group was extremely significantly higher(P<0.01)than that of control group,ACC1 mRNA relative expression quantity of 0.2μmol/L group and 0.5μmol/L group was significantly higher(P<0.05)than that of control group.When the test time was 48 h,GLUT-4 mRNA relative expression quantity of 1μmol/L group was significantly(P<0.05)lower than that of control group,PK mRNA relative expression quantity of 1μmol/L group was extremely significantly(P<0.01)higher than that of control group,FAS mRNA relative expression quantity of 0.5μmol/L group was significantly(P<0.05)higher than that of 1μmol/L group,FAS mRNA relative expression quantity of 1μmol/L group was significantly(P<0.05)higher than that of control group,ACC1 mRNA relative expression quantity of 1μmol/L group was extremely significantly(P<0.01)higher than that of other groups.【Conclusion】The addition of biotin can inhibit the

关 键 词：生物素 3T3-L1脂肪细胞 脂合成 

分 类 号：Q253[生物学—细胞生物学] S188[农业科学—农业基础科学]