姜黄素新型纳米乳药动学与生物等效性研究  

Study on Pharmacokinetics and Bioequivalence of Curcumin Novel Nano Emulsion in Rats

作　　者：雷婷婷[1] 张景勍[1] 张雪[1] 张严方[1] 赵华[1] LEI Tingling;ZHANG Jingqing;ZHANG Xue;ZHANG Yanfang;ZHAO Hua(Chongqing Research Center for Pharmaceutical Engineering,Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]重庆医科大学重庆药物高校工程研究中心,重庆400016

出　　处：《食品与生物技术学报》2018年第2期146-152,共7页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(30973645);重庆市首批高等学校优秀人才资助计划项目(渝教人(2009)2号文件)

摘　　要：建立高效液相色谱法(HPLC)测姜黄素新型纳米乳(Curcumin novel nano emulsion,CNNE)中姜黄素(Curcumin,Cur)在大鼠体内的血药浓度,通过比较相同给药计量下Cur和CNNE在大鼠体内的血药浓度考察CNNE的药代动力学行为,并比较二者生物等效性。采用水滴法制备CNNE,灌胃给予CNNE与Cur后,采用HPLC法测定Cur的血药浓度,绘制药物浓度物-时间曲线(AUC),DAS2.1.1软件计算药代参数与生物等效性。CNNE与Cur的房室模型AUC(0-∞)分别为(3 439.27±180.63)(μg·h)/L、(482.07±42.14)(μg·h)/L,CNNE与Cur的非房室模型AUC(0-∞)分别为(3 410.20±154.09)(μg·h)/L、(446.66±44.02)(μg·h)/L,CNNE的相对生物利用度为713.44%、763.49%。结果表明,将Cur制备成CNNE后促进了Cur的吸收,CNNE的生物利用度提高了约7倍。CNNE与Cur具有生物不等效性。To build a method that Curcumin(Cur)of Curcumin novel nano emulsion,CNNE)were measured by HPLC,study the pharmacokinetic characteristics of the CNNE,and compare the bioequiavailability of CNNE and Cur.CNNE was prepared by water droplets method,and plasma drug concentrations of different time points were measured by HPLC after oral administration CNNE and Cur.The curve of AUC was drew by excel software,and the main pharmacokinetic parameters and bioequiavailability were calculated by DAS2.1.1 software.The parameters of compartmental model,AUC(0-∞)of CNNE and Cur,were(3 439.27±180.63)(μg·h)/L,(482.07±42.14)(μg·h)/L,and the parameters of compartmental model,AUC(0-∞)of CNNE and Cur,were(3 410.20±154.09)(μg·h)/L,(446.66±44.02)(μg·h)/L.The relative bioavailability of compartmental model and non-compartmental of CNNE are 713.44%,763.49%,respectively.The results show that prepared CNNE promoted absorption of Cur,and the bioavailability of CNNE is about seven times as much as Cur.CNNE and Cur are not bioequivalent.

关 键 词：姜黄素 羟丙基β-环糊精 磷脂 纳米乳 药动学 

分 类 号：Q556[生物学—生物化学] R392-33[医药卫生—免疫学]