牛奶中残留麻保沙星的微生物学检测方法  

Determination of Marbofloxacin Residues in Milk by Microbiological Method

作　　者：陈秀金[1] 任国艳[1] 许凯旋 崔璨 张永丽 侯玉泽[1] 胥传来[2] 李根 CHEN Xiujin;REN Guoyan;XU Kaixuan;CUI Can;ZHANG Yongli;HOU Yuze;XU Chuanlai;LI Gen(Food and Bioengineering Institute,Henan University of Science and Technology,Luoyang 471023;School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471023 [2]江南大学食品学院,江苏无锡214122

出　　处：《食品与生物技术学报》2018年第1期107-111,共5页Journal of Food Science and Biotechnology

基　　金：河南省高等学校重点科研项目计划(15A550003);河南科技大学博士科研启动基金(09001789);河南科技大学大学训练计划(2014116)资助

摘　　要：建立牛奶中残留麻保沙星的微生物学检测方法。试验以金黄色葡萄球菌为敏感菌,采用滤纸片法进行测定,以牛奶为样设计了添加回收试验以证实该方法的有效性。磷酸盐缓冲溶液和牛奶基质麻保沙星标准曲线的工作范围是0.1~1.6μg/mL,最低检测限为0.1μg/mL。对纯牛奶样的3种添加水平,平均回收率范围在77.3%~93.3%之间,变异系数小于7.26%,该方法可以用于牛奶中麻保沙星残留的检测。To establish a microbiological method for determination of marbofloxacin residues in milk.Marbofloxacin residues in milk was tested using filter paper method based on Staphylococcus selected as a sensitive bacteria,and then the recoveries were performed on milk samples to verify the feasibility of this method.Results:the data showed that the linear range for mornitoring marbofloxacin was from 0.1μg/mL to 1.6μg/mL based on phosphate buffer solution and milk matrix.The minimum limit detections of marbofloxacin in phosphate buffer solution and milk matrix were 0.1μg/mL.The average recovery rates were in the range of 77.3%~93.3%and variation coefficient were less than 7.26%for three spiked levels of Erie milk samples.The obtained method can be feasible to mornitoring marbofloxacin residues in milk.

关 键 词：麻保沙星 牛奶 滤纸片法 残留检测 

分 类 号：S859.84[农业科学—临床兽医学]