PBNA-413促进细胞自噬保护缺血/再灌注损伤心肌的研究  

A Study on the Protective Effects of PBNA-413 in Myocardial Ischemia Reperfusionin Rats by Promoting Cell Autophagy

作　　者：曾丽梅 甘钰[3] 胡鸿 李涛 黄帝锡[3] 马能军 黎晓 黄志华[2] ZENG Li-mei;GAN Yu;HU Hong;LI Tao;HUANG Di-xi;MA Neng-jun;LI Xiao;HUANG Zhi-hua(Jiangxi University of Traditional Chinese Medicine,Nanchang,Jiangxi 330000;School of Basic Medical Science,Gannan Medical University,Ganzhou,Jiangxi 341000;Undergraduate Student,Grade 2014,Gannan Medical University,Ganzhou,Jiangxi 341000)

机构地区：[1]江西中医药大学,江西南昌330000 [2]赣南医学院基础医学院,江西赣州341000 [3]赣南医学院,江西赣州341000

出　　处：《赣南医学院学报》2018年第2期103-107,共5页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：江西省自然科学基金项目(20122BAB205039)

摘　　要：目的:探讨拳参正丁醇提取物-413(Polygonum Bistorla L.n-Butyl Alcohol Extract,PBNA-413)通过调节自噬对大鼠心肌缺血/再灌注损伤的保护作用。方法:将40只雄性大鼠随机分为5组:假手术组、I/R模型组,PBNA-413低、中、高(0.1、0.3、1 mg·kg^(-1))剂量组。结扎左冠状动脉前降支使心肌缺血45 min,再灌注1 h,复制缺血/再灌注损伤模型,并记录心电图。取心肌组织,采用q-PCR方法和蛋白免疫印迹法检测心肌组织Beclin-1、LC3Ⅱ的mRNA表达及蛋白表达情况。结果:结扎左冠状动脉前降支后,心电图示ST段明显抬高,PBNA-413呈剂量依赖性降低ST段,以高剂量的效果最好。与假手术组比较,模型组大鼠心肌细胞内Beclin-1、LC3Ⅱ蛋白表达降低;与I/R模型组相比,PBNA-413高剂量组(1.0 mg·kg^(-1))Beclin-1、LC3Ⅱ表达升高。LC3Ⅱ、Beclin-1的基因水平变化不明显。结论:PBNA-413可能通过适度促进细胞自噬从而有效保护缺血/再灌注损伤心肌。Objective:To investigate the protective effect of Polygonum Bistorla L.n-Butyl Alcohol Extract 413(PBNA-413)on myocardial ischemia-reperfusion injury in rats by regulating autophagy.Methods:Totally 40 male SD rats were randomly divided into five groups:the control group(sham),the ischemia reperfusion group(vehicle),the high,middle and low dose(1.0,0.3,0.1 mg·kg-1)PBNA-413 treatment group.The models of MI/R injury in rats were created through ligating the left anterior descending coronary artery for 45 min and reperfusing for 60 min.In the process,the electrocardiogram(ECG)was recorded continuously.The expression of autophagy-related proteins and genes,such as Beclin-1 and LC3II were detected by quantitative polymerase chain reaction(q-PCR)and Western blotting assay.Results:After the ligation of anterior descending branch of left coronary artery,the ECG ST segment was significantly elevated,and PBNA-413 showed a dose dependence reduction of ST segment,with the best results at high dose.Compared with sham group,the expression of Beclin-1 and LC3II protein in cardiac myocytes was decreased in MI/R model group,while the gene levels of Beclin-1 and LC3II had no obvious changes.Compared with MI/R model group,the PBNA-413 high dose group showed an increased expression in the protein level of Beclin-1 and LC3II.Conclusion:PBNA-413 may protect myocardium from ischemia/reperfusion injury by appropriately promoting autophagy.

关 键 词：拳参正丁醇提取物-413 心肌保护 心肌缺血/再灌注 细胞自噬 

分 类 号：R285[医药卫生—中药学]