IL-17D调控肺脏NK细胞募集及黄芪的促进作用  

IL-17D regulates recruitment of lung NK cells and promotion of Astragalus membranaceus

作　　者：兰红云[1] 郑晓丹[1,2] 郭钰琪 安磊[1,4] 孙晓艳[1,3] 吕衍民 王丽 姚成芳[1,3] LAN Hong-Yun;ZHENG Xiao-Dan;GUO Yu-Qi;AN Lei;SUN Xiao-Yan;Lü Yan-Min;WANG Li;YAO Cheng-Fang(School of Medicine and Life Sciences,University of Jinan&Shandong Academy of Medical Sciences,Jinan 250200,China)

机构地区：[1]济南大学,山东省医学科学院医学与生命科学学院,济南250200 [2]湖北省汉口医院检验科,武汉430000 [3]山东省医学科学院基础医学研究所,济南250062 [4]山东航空股份有限公司综合保障部航卫中心医务室,济南250107

出　　处：《中国免疫学杂志》2018年第3期393-397,共5页Chinese Journal of Immunology

基　　金：本文为国家自然科学基金资助项目(81573728)、山东省自然科学基金资助项目(ZR2015HM007)和山东省医药卫生科技发展计划项目(2015WS0192)。

摘　　要：目的:探讨IL-17D调控肺脏NK细胞的募集机制及中药黄芪的促进作用。方法:采用尾静脉注射B16黑色素瘤细胞建立小鼠肺肿瘤转移模型,分别经IL-17D或黄芪处理后,应用流式细胞术和RT-PCR法检测各组小鼠肺脏IL-17D表达状态及其NK细胞含量的变化。结果:小鼠肺转移瘤模型中IL-17D表达水平及其肺脏NK细胞含量急剧下降,经IL-17D处理后可明显上调肺脏NK细胞含量、降低肺脏肿瘤克隆形成。同时,CXCL9、IL-15等与NK募集和功能相关的细胞因子表达明显上调。中药黄芪可促进肺脏IL-17D表达水平,同时上调肺脏NK细胞含量,抑制肺脏肿瘤克隆的形成。结论:IL-17D可调控NK细胞的肺脏募集;中药黄芪可通过上调IL-17D的表达,从而增强NK的肺脏募集能力,促进肺脏抗肿瘤免疫效应。Objective:To investigate the role of IL-17D to regulate the recruitment of lung NK cells and the effects of Astragalus membranaceus on IL-17D.Methods:The lung metastasis of tumor model was established by injecting B16 melanoma cells through caudal vein.After being treated with IL-17D or Astragalus membranaceus respectively,the expression of IL-17D and NK cells in lung was detected by flow cytometry and RT-PCR.Results:The production of IL-17D and the content of NK cells in the lung tumor metastasis model were dramatically decreased.After treatment with IL-17D,the content of NK cells in lung was significantly increased and the lung tumor foci were decreased.Along with up-regulated recruitments of NK cells,CXCL9 and IL-15,which support NK cell recruitments and maintain survival,were significantly increased.Astragalus inhibited lung tumor developments in lung by increasing the IL-17D expression and up-regulated the lung NK cell content.Conclusion:IL-17D can regulate the lung recruitment of NK cells.Astragalus mongholicus can enhance the capacity of NK cell recruitment and promote anti-tumor immune effects by up-regulating the expression of IL-17D.

关 键 词：IL-17D NK细胞 肺脏 黄芪 肺癌 

分 类 号：R392.5[医药卫生—免疫学]