精神分裂症模型鼠腹腔中的中性粒细胞趋化运动的动态可视化分析  

Dynamic visual analysis of neutrophils chemotaxis in peritoneal cavity of schizophrenic model in mice

作　　者：白宁 杨凌飞 安丽华 王雯 李昀倩 盛荟 王彤 李华侃 袁兰 BAI Ning;YANG Ling-fei;AN Li-hua;WANG wen;LI Yun-qian;SHENG Hui;WANG Tong;LI Hua-kan;YUAN Lan(Department of Pharmceutical,Jinzhou Medical University,Jinzhou 121000,Liaoning,China;Peking University Medical and Health Analysis Center,Beijing 100191,China;Department of Chemical Biology,Peking University School of Parmaceutical Sciences,Beijing 100191,China;Department of Basic Science,Jinzhou Medical University,Jinzhou 121000,Liaoning,China)

机构地区：[1]锦州医科大学药学院,辽宁锦州121000 [2]北京大学医药卫生分析中心,北京100191 [3]北京大学药学院化学生物学系,北京100191 [4]锦州医科大学公共基础学院,辽宁锦州121000

出　　处：《北京大学学报（医学版）》2018年第2期226-230,共5页Journal of Peking University:Health Sciences

基　　金：国家重点研发计划(2016YFC0103605;2016YFC0103602;2016YFC0103600);国家自然科学基金(91330103;91630314;81471633);国家杰出青年基金(61625102);北京市科技计划(Z16100000116041)资助~~

摘　　要：目的:应用细胞动态可视化系统观察并分析正常鼠和精神分裂症模型鼠腹腔中的中性粒细胞趋化运动能力及其差异。方法:18只健康昆明小鼠随机分为对照组(6只)、0.3 mg/kg 5-甲基二氢二苯并环庚烯亚氨马来酸(dizocilpine maleate,MK-801)处理组(6只)、0.6 mg/kg MK-801处理组(6只),分别提取中性粒细胞,在显微镜下观察形态并计数。将每组细胞分成两份进行趋化实验,分别为趋化剂处理组和趋化剂未处理组,即对照组1、0.3 mg/kg MK-801处理组1、0.6 mg/kg MK-801处理组1和对照组2、0.3 mg/kg MK-801处理组2、0.6 mg/kg MK-801处理组2。使用NIS-Elements软件记录细胞动态迁移过程。使用TAXIScan Analyzer 2软件每组细胞随机选取30个细胞(即n=30)分析细胞迁移的轨迹、速度和距离,并采用配对检验和单因素方差分析进行统计学分析。结果:对照组、0.3 mg/kg MK-801处理组、0.6 mg/kg MK-801处理组的中性粒细胞的数量分别是(1.00±0.03)×104/m L、(0.05±0.02)×104/m L、(0.32±0.01)×104/m L,差异均有统计学意义(P<0.05)。在趋化剂的作用下,对照组1、0.3 mg/kg MK-801处理组1、0.6 mg/kg MK-801处理组1的中性粒细胞定向迁移能力分别是(0.85±0.11)radian、(1.00±0.11)radian、(0.96±0.10)radian,差异均有统计学意义(P<0.05);中性粒细胞迁移速度分别是(0.09±0.02)μm/s、(0.12±0.01)μm/s、(0.14±0.01)μm/s,差异均有统计学意义(P<0.05);中性粒细胞迁移距离分别是(94.26±0.02)μm、(134.61±0.01)μm、(156.19±0.01)μm,差异均有统计学意义(P<0.05)。结论:与对照组腹腔中的中性粒细胞比较,精神分裂症模型鼠腹腔中的中性粒细胞具有更强的趋化运动能力。Objective:To compare the differences of neutrophils chemotaxis ability in peritoneal cavity between normal rats and schizopherenic rats with cell dynamic visualization system.Methods:In the study,18 healthy Kunming rats were randomly divided into 3 groups which were control group(n=6),0.3 mg/kg MK-801 treatment group(n=6),0.6 mg/kg dizocilpine maleate(MK-801)treatment group(n=6),extracted neutrophils separately,and observed the morphology and counted under a microscope.Each group of cells was divided into two parts for chemotactic experiment,called chemokine agent treatment group and no chemokine agent treatment group respectively,indicating control 1,0.3 mg/kg MK-801 treatment 1,0.6 mg/kg MK-801 treatment 1 and control 2,0.3 mg/kg MK-801 treatment 2,0.6 mg/kg MK-801 treatment 2.The dynamic migration of cells was recorded using the NIS-Elements software,and TAXIScan Analyzer 2 software was used to select 30 cells(n=30)in each group of cells and analyze cells migration trajectory,speed and distance,and use pair test and One-Way analysis of variance for statistical analysis.Results:The number of neutrophils in control group,0.3 mg/kg MK-801 treatment group and 0.6 mg/kg MK-801 treatment group were(1.00±0.03)×104/mL,(0.05±0.02)×104/mL,(0.32±0.01)×104/mL respectively,the differences of results were statistically significant(P<0.05).Under the effect of chemotactic agent,the directional migration capability of neutrophils in control group 1,0.3 mg/kg MK-801 treatment group 1 and 0.6 mg/kg MK-801 treatment group 1 were(0.85±0.11)radian,(1.00±0.11)radian,(0.96±0.10)radian respectively(P<0.05);the migration velocities of neutrophils were(0.09±0.02)μm/s,(0.12±0.01)μm/s,(0.14±0.01)μm/s respectively(P<0.05);the migration distances of neutrophils were(94.26±0.02)μm,(134.61±0.01)μm,(156.19±0.01)μm respectively(P<0.05).Conclusion:Compared with neutrophils in peritoneal cavity of control group,the neutrophils in peritoneal cavity of schizophrenic rats have stronger chemotactic movement ability.

关 键 词：精神分裂症 中性粒细胞 细胞趋化运动 

分 类 号：R122.11[医药卫生—环境卫生学]